Bacterial community structure and succession in nests of two megachilid bee genera
Bacterial community structure and succession in nests of two megachilid bee genera
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DOI：
10.1093/femsec/fiy218
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发表时间：
2018-10
影响因子：
4.2
通讯作者：
A. Voulgari-Kokota;G. Grimmer;I. Steffan‐Dewenter;A. Keller
中科院分区：
文献类型：
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作者：
A. Voulgari-Kokota;G. Grimmer;I. Steffan‐Dewenter;A. Keller
&NA;对蜜蜂的研究揭示了细菌类群在蜂巢中发挥着关键功能，包括营养吸收和免疫反应。尽管独居蜜蜂提供了宝贵的生态服务，但其微生物群落对幼虫健康以及成虫的发育和健康的贡献主要未知。为了解决这一差距，我们进行了一项 16S rDNA 元条形码研究，包括来自两个不同的巨幼独居蜂属的幼虫和巢室中储存的花粉。我们测试了宿主分类、环境背景和幼虫的发育阶段如何决定相关细菌群落的丰富度和组成。总共对来自二角壁蜂、蓝壁壁蜂、圆叶蜂和花叶蜂巢的 198 个标本进行了研究。筑巢环境中的独居蜂细菌微生物群在物种内大多是同质的，并且不受景观的显着影响。对于每种蜜蜂物种，我们鉴定了在幼虫和储存的花粉中表现出一致的细菌类群。对于花粉供应，我们还描述了随着幼虫发育的进展而发生的群落转变，表明输入的花细菌减少。 &NA;图形抽象图。我们调查了不同景观中四种独居蜂种的幼虫和花粉供给，确定了重复出现的细菌伙伴、物种特异性群落以及随着幼虫发育的进展而发生的变化
&NA; Studies on honeybees have revealed bacterial taxa which adopt key functions in the hive, in terms of nutrient uptake and immune responses. Despite solitary bees providing invaluable ecological services, the contribution of their microbial communities to larval health and the development and fitness of adults is mainly unknown. To address this gap, we conducted a 16S rDNA meta‐barcoding study including larvae and stored pollen in nest chambers from two different megachilid solitary bee genera. We tested how host taxonomy, environmental context and the developmental stage of larvae determined richness and composition of associated bacterial communities. A total of 198 specimens from Osmia bicornis, Osmia caerulescens, Megachile rotundata and Megachile versicolor nests were investigated. Solitary bee bacterial microbiota in the nesting environment were mostly homogeneous within species, and not significantly affected by landscape. For each bee species, we identified bacterial taxa that showed consistent occurrence in the larvae and stored pollen. For the pollen provision, we also described a community shift with progressing larval development, suggesting a reduction of imported floral bacteria. &NA; Graphical Abstract Figure. We investigated larvae and pollen provisions from four solitary bee species in different landscapes, identified reoccurring bacterial associates, species‐specific communities as well as shifts in line with progressing larval development