Lost and found: Frogs in a biodiversity hotspot rediscovered with environmental DNA
Lost and found: Frogs in a biodiversity hotspot rediscovered with environmental DNA
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DOI：
10.1111/mec.15594
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发表时间：
2020-09-03
影响因子：
4.9
通讯作者：
Zamudio, Kelly Raquel
中科院分区：
文献类型：
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作者：
Lopes, Carla Martins;Baeta, Delio;Zamudio, Kelly Raquel
下降和灭绝在全球范围内增加，并挑战保护主义者，以跟上生物多样性监测的步伐。有机体在环境中留下DNA痕迹，例如在土壤，水和空气中。这些DNA痕迹称为环境DNA（EDNA）。 EDNA的分析是一种高度敏感的方法，具有快速评估局部多样性和受威胁物种状况的潜力。我们使用环境DNA Metabarcoding方法以及广泛的序列参考数据库搜索了30种目标两栖动物物种的DNA痕迹，在不同级别的威胁下，在不同级别的威胁范围内搜索了DNA痕迹，以分析来自大西洋沿海森林和巴西邻近塞拉多草地的六个蒙塔尼地点的水样。我们成功地检测了四个衰落的物种的DNA痕迹（Hylodes Ornatus，Hylodes Regius，Crossodactylus timbuhy，Andvitreorana eurygnatha）；两个局部消失了（phasmahyla exilisandandphasmahyla guttata）；以及自1968年以来一直没有看到的一种物种（被预定的托梅格罗西亚玻果素）。我们证实了没有传统方法未发现的物种，强调了Edna Metabarcoding在低种群密度下，尤其是在Megadiverse热带地点的生物多样性监测的疗效。我们的结果支持Edna在保护生物学中的潜在应用，以评估受调查栖息地或地点中受威胁物种的持久性和分布，并提高红色清单的准确性，尤其是对于长期未被发现的物种。
Declines and extinctions are increasing globally and challenge conservationists to keep pace with biodiversity monitoring. Organisms leave DNA traces in the environment, e.g., in soil, water, and air. These DNA traces are referred to as environmental DNA (eDNA). The analysis of eDNA is a highly sensitive method with the potential to rapidly assess local diversity and the status of threatened species. We searched for DNA traces of 30 target amphibian species of conservation concern, at different levels of threat, using an environmental DNA metabarcoding approach, together with an extensive sequence reference database to analyse water samples from six montane sites in the Atlantic Coastal Forest and adjacent Cerrado grasslands of Brazil. We successfully detected DNA traces of four declined species (Hylodes ornatus,Hylodes regius,Crossodactylus timbuhy, andVitreorana eurygnatha); two locally disappeared (Phasmahyla exilisandPhasmahyla guttata); and one species that has not been seen since 1968 (putatively assigned toMegaelosia bocainensis). We confirm the presence of species undetected by traditional methods, underscoring the efficacy of eDNA metabarcoding for biodiversity monitoring at low population densities, especially in megadiverse tropical sites. Our results support the potential application of eDNA in conservation biology, to evaluate persistence and distribution of threatened species in surveyed habitats or sites, and improve accuracy of red lists, especially for species undetected over long periods.