New and emerging uses of CRISPR/Cas9 to genetically manipulate apicomplexan parasites.
New and emerging uses of CRISPR/Cas9 to genetically manipulate apicomplexan parasites.
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DOI：
10.1017/s003118201800001x
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发表时间：
2018-08
期刊：
影响因子：
2.4
通讯作者：
Carruthers VB
中科院分区：
文献类型：
--
作者：
Di Cristina M;Carruthers VB
尽管CRISPR/Cas9基因组工程方法的应用仅在3年前才首次在顶复门寄生虫中报道，但该技术已迅速成为顶复门寄生虫研究的重要组成部分。这篇综述简要介绍了CRISPR/Cas9的历史及其使用背后的原理，沿着记录了其在顶复门寄生虫，特别是疟原虫中的实施。和弓形虫。我们还讨论了最近使用CRISPR/Cas9在T.并强调其用于隐孢子虫属的精液遗传操作。最后，我们考虑了尚未在顶复门中实施的CRISPR/Cas9的新变体。尽管CRISPR/Cas9已经加速了对顶复门动物基因功能的快速研究，但随着新的变化和创新被整合到该领域，这项技术的全部潜力还有待实现。
Although the application of CRISPR/Cas9 genome engineering approaches was first reported in apicomplexan parasites only 3 years ago, this technology has rapidly become an essential component of research on apicomplexan parasites. This review briefly describes the history of CRISPR/Cas9 and the principles behind its use along with documenting its implementation in apicomplexan parasites, especially Plasmodium spp. and Toxoplasma gondii. We also discuss the recent use of CRISPR/Cas9 for whole genome screening of gene knockout mutants in T. gondii and highlight its use for seminal genetic manipulations of Cryptosporidium spp. Finally, we consider new variations of CRISPR/Cas9 that have yet to be implemented in apicomplexans. Whereas CRISPR/Cas9 has already accelerated rapid interrogation of gene function in apicomplexans, the full potential of this technology is yet to be realized as new variations and innovations are integrated into the field.