Identification of the C-elegans anaphase promoting complex subunit Cdc26 by phenotypic profiling and functional rescue in yeast
Identification of the C-elegans anaphase promoting complex subunit Cdc26 by phenotypic profiling and functional rescue in yeast
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DOI：
10.1186/1471-213x-7-19
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发表时间：
2007-03-20
影响因子：
--
通讯作者：
Ahringer, Julie
中科院分区：
文献类型：
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作者：
Dong, Yan;Bogdanova, Aliona;Ahringer, Julie
背景：RNA干扰结合线虫胚胎的视频记录是鉴定参与细胞分裂过程的基因的一种有效方法。结果：B0511.9基因的弱RNAi抑制作用会导致胚胎细胞极性缺陷，而强抑制作用会导致胚胎在减数分裂I中期停滞。B0511.9的RNAi诱导的缺陷范围与后期促进复合体/环体(APC/C)组分的突变体所显示的缺陷范围惊人地相似。虽然相似性搜索没有发现B0511.9在非冗余蛋白质数据库中有任何明显的同源性，但我们发现该N端与小的发芽酵母APC/C亚基CDC26的N端及其来自其他各种生物的同源物具有保守的序列模式。此外，我们发现B0511.9有力地补充了酵母cdc26对温度敏感的生长缺陷。结论：B0511.9编码线虫APC/C亚基CDC26。
Background: RNA interference coupled with videorecording of C. elegans embryos is a powerful method for identifying genes involved in cell division processes. Here we present a functional analysis of the gene B0511.9, previously identified as a candidate cell polarity gene in an RNAi videorecording screen of chromosome I embryonic lethal genes.Results: Whereas weak RNAi inhibition of B0511.9 causes embryonic cell polarity defects, strong inhibition causes embryos to arrest in metaphase of meiosis I. The range of defects induced by RNAi of B0511.9 is strikingly similar to those displayed by mutants of anaphase-promoting complex/cyclosome ( APC/C) components. Although similarity searches did not reveal any obvious homologue of B0511.9 in the non-redundant protein database, we found that the N-terminus shares a conserved sequence pattern with the N-terminus of the small budding yeast APC/C subunit Cdc26 and its orthologues from a variety of other organisms. Furthermore, we show that B0511.9 robustly complements the temperature-sensitive growth defect of a yeast cdc26. mutant.Conclusion: These data demonstrate that B0511.9 encodes the C. elegans APC/C subunit CDC26.