Regional crypt function in rat large intestine in relation to fluid absorption and growth of the pericryptal sheath
Regional crypt function in rat large intestine in relation to fluid absorption and growth of the pericryptal sheath
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大鼠大肠的区域隐窝功能与液体吸收和隐窝周围鞘的生长相关
DOI：
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Kevin C. Pedley
中科院分区：
文献类型：
--
作者：
R. Naftalin;Kevin C. Pedley
1对大鼠结肠粘膜的共聚焦显微镜研究表明，远端结肠隐窝周围的隐窝周鞘是葡聚糖和NaCl运动的有效屏障，而盲肠隐窝周围没有这样的结构。（2）钠绿色在远端结肠的周围间隙内的积聚，证实了远端结肠的周围屏障的功能。苯扎明暴露后，钠绿色积累减少。异硫氰酸荧光素标记的葡聚糖（FITC葡聚糖;分子量10000 Da）在隐窝腔和隐窝周围空间中积聚。盲肠隐窝周围的隐周区不存在葡聚糖和钠绿色蓄积。3低饮食Na+摄入量升高大鼠血浆醛固酮并刺激远端隐周鞘生长和增殖，如隐周带中F‐actin、平滑肌肌动蛋白、β‐catenin和E‐cadherins数量增加所示。它还提高了远端结肠抵抗高管腔液压阻力的能力。这种联系表明，营养对结肠的影响，从低钠+饮食不仅限于对跨上皮Na+运输的影响，但观察到的pericryptal鞘。4.隐窝功能的计算机模型证实，对NaCl具有低渗透性的隐窝周鞘是隐窝脱水机制的重要组成部分。
1 Confocal microscopic studies of rat colonic mucosa showed that the pericryptal sheath surrounding distal colonic crypts is an effective barrier both to dextran and NaCl movement, whereas no such structure surrounds the caecal crypts. 2 The distal colonic pericryptal barrier was functionally demonstrated by accumulation of Sodium Green within the pericryptal space. After exposure to benzamil, Sodium Green accumulation was decreased. Fluorescein isocyanate‐labelled dextran (FITC dextran; molecular mass 10000 Da) was accumulated in the crypt lumens and pericryptal spaces. Both dextran and Sodium Green accumulation were absent from the pericryptal zone surrounding caecal crypts. 3 Low dietary Na+ intake raised rat plasma aldosterone and stimulated distal pericryptal sheath growth and adhesiveness as shown by increased amounts of F‐actin, smooth muscle actin, β‐catenin and E‐cadherins in the pericryptal zone. It also raised the capacity of the distal colon to dehydrate against a high luminal hydraulic resistance. This linkage indicates that trophic effects on the colon resulting from a low Na+ diet are not confined solely to effects on transepithelial Na+ transport, but are observed in the pericryptal sheath. 4 A computer model of crypt function confirms that a pericryptal sheath with low permeability to NaCl is an essential component of the crypt dehydrating mechanism.
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影响因子：
4.4
作者：
S. Petridou;S. Masur
通讯作者：
S. Petridou;S. Masur
兔近端结肠：独特的运输上皮。
DOI：
10.1152/ajpgi.1984.246.5.g603
发表时间：
1984
期刊：
The American journal of physiology
影响因子：
--
作者：
Sellin,JH;DeSoignie,R
通讯作者：
DeSoignie,R
DOI：
10.1016/s0022-2828(95)90359-3
发表时间：
1995-08-01
影响因子：
5
作者：
CAMPBELL, SE;JANICKI, JS;WEBER, KT
通讯作者：
WEBER, KT
阿米洛利类似物抑制结肠钠转运。
DOI：
10.1152/ajpcell.1989.256.1.c67
发表时间：
1989
期刊：
The American journal of physiology
影响因子：
--
作者：
Bridges,RJ;CragoeJr,EJ;Frizzell,RA;Benos,DJ
通讯作者：
Benos,DJ