Sample Preparation for Relative Quantitation of Proteins Using Tandem Mass Tags (TMT) and Mass Spectrometry (MS).
Sample Preparation for Relative Quantitation of Proteins Using Tandem Mass Tags (TMT) and Mass Spectrometry (MS).
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使用串联质量标签（TMT）和质谱法（MS）对蛋白质相对定量的样品制备。
DOI：
10.1007/978-1-4939-7659-1_11
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Neubert TA
中科院分区：
文献类型：
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作者：
Erdjument-Bromage H;Huang FK;Neubert TA
定量蛋白质组分析允许在多种细胞类型或条件下比较蛋白质或磷蛋白水平。定量蛋白质组学的一些实验方法已经被描述。在本章中，我们重点介绍了肽的串联质量标签（TMT）等压标记，用于蛋白质和磷酸肽的全局相对定量。迄今为止，还没有公开的方案描述从小肿瘤样本中特异性提取的少量肽的化学标记，为此需要严格的样品制备以确保可重复的TMT标记。
Quantitative proteome analysis allows comparisons of protein or phosphoprotein levels across multiple cell types or conditions. A number of experimental approaches have been described toward quantitative proteomics. In this chapter, we focus on Tandem Mass Tag (TMT) isobaric labeling of peptides for global, relative quantitation of proteins and phosphopeptides. To date, there has been no published protocol describing chemical labeling of small amounts of peptides specifically extracted from small tumor samples, for which rigorous sample preparation is necessary to ensure reproducible TMT labeling.