Study on differentiation of chicken spermatogonial stem cells into osteoblasts in vitro
Study on differentiation of chicken spermatogonial stem cells into osteoblasts in vitro
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发表时间：
2007
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通讯作者：
Liu Bi-chun
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作者：
Liu Bi-chun
本研究旨在探讨精原干细胞（SSCs）在体外向成骨细胞分化的能力。在鸡胚第18 ~20天从鸡胚睾丸中分离ssc，进行体外培养和传代培养。用去地塞米松、2-磷酸甘油二钠水合盐和维生素C诱导ssc形成成骨细胞，采用Von Kossa染色、细胞化学染色和免疫组织化学染色检测。结果表明，诱导15~21 d后，成骨细胞从SSCs分化而来，阳性细胞率为75%~80%。Von Kossa染色示分化细胞内分布黑色颗粒，提示细胞内矿物积聚。ALP和免疫组织化学染色也证实了我们的结果。综上所述，我们的研究结果表明，鸡ssc可以向成骨细胞分化，表明ssc在体外具有多能分化的能力。
The current project is conducted to investigate the capability of spermatogonial stem cells(SSCs) differentiating into osteoblasts in vitro.SSCs were isolated from chicken embryo testis on 18th~20th hatching days,then cultured and subcultured in vitro.SSCs were induced into osteoblasts by desamethason,glycerol 2-phosphate disodium salt hydrate and vitamin C and examined by Von Kossa's stain,cytochemistry staining and immunohistochemistry staining.The results showed that osteoblasts were differentiated from SSCs after 15~21 days of induction and with 75%~80% of positive cells efficiency.Black granules were distributed in the differentiated cells by Von Kossa's staining,suggesting mineral cumulation in the cells.Our results were also confirmed by ALP and immunohistochemistry staining.Together,our results indicated that the chicken SSCs could be differentiated into osteoblasts,suggesting SSCs have the capability of multipotential differentiation in vitro.