Effects of ovariectomy on aggregation, secretion, and metalloproteinases in porcine platelets.
Effects of ovariectomy on aggregation, secretion, and metalloproteinases in porcine platelets.
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卵巢切除术对猪血小板聚集、分泌和金属蛋白酶的影响。
DOI：
10.1152/ajpheart.00958.2002
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Miller,VirginiaM
中科院分区：
文献类型：
--
作者：
Jayachandran,Muthuvel;Owen,WhyteG;Miller,VirginiaM
卵巢激素缺失后生长因子（包括基质金属蛋白酶（MMPs））聚集和释放的差异可能导致卵巢切除动物对动脉损伤的过度反应。因此，本实验旨在比较性成熟（7月龄）和去卵巢（4周）母猪循环血小板中基质蛋白酶（MMP-2、MMP-9和MMP-14）和金属蛋白酶组织抑制剂（TIMP-1和TIMP-2）的聚集、致密颗粒ATP释放、表达。卵巢切除术后循环血小板数量没有变化，但网状血小板的百分比明显增加。卵巢切除术后血小板聚集和致密颗粒状ATP分泌也显著增加。在血小板裂解物中，卵巢切除术后活性MMP-2增加，而MMP-14显著降低；TIMP-1、TIMP-2和p -选择素的表达没有变化。这些结果提示卵巢切除术后血小板周转、聚集和ATP分泌增加。此外，卵巢激素选择性调节MMPs在猪血小板中的表达和活性。血小板聚集和MMP-2活性的增加会改变血小板-血小板和血小板-血管壁的相互作用，导致卵巢激素丧失对损伤的过度反应。
Differences in the aggregation and release of growth factors including matrix metalloproteinases (MMPs) after loss of ovarian hormones could contribute to an exaggerated response to injury in arteries of ovariectomized animals. Therefore, experiments were designed to compare aggregation, dense granular ATP release, expression of MMPs (MMP-2, MMP-9, and MMP-14) and tissue inhibitors of metalloproteinase (TIMP-1 and TIMP-2) in circulating platelets from sexually mature (7 mo old) gonadally intact and ovariectomized (4 wk) female pigs. Numbers of circulating platelets did not change after ovariectomy, but the percentage of reticulated platelets increased significantly. Platelet aggregation and dense granular ATP secretion also increased significantly with ovariectomy. In platelet lysates, active MMP-2 increased, whereas MMP-14 significantly decreased, after ovariectomy; the expression of TIMP-1, TIMP-2, and P-selectin did not change. These results suggest that platelet turnover, aggregation, and ATP secretion increase with ovariectomy. Also, ovarian hormones selectively regulate the expression and activity of MMPs in porcine platelets. Increased platelet aggregation and activity of MMP-2 would alter platelet-platelet and platelet-vessel wall interactions, contributing to an exaggerated response to injury with loss of ovarian hormones.