Barcoding animal life:: cytochrome c oxidase subunit 1 divergences among closely related species
Barcoding animal life:: cytochrome c oxidase subunit 1 divergences among closely related species
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DOI：
10.1098/rsbl.2003.0025
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发表时间：
2003-08-07
影响因子：
4.7
通讯作者：
deWaard, JR
中科院分区：
文献类型：
--
作者：
Hebert, PDN;Ratnasingham, S;deWaard, JR
动物王国拥有数百万个物种及其生命阶段的转变，为分类学提供了一个具有挑战性的目标。最近的工作表明，基于线粒体基因，细胞色素c氧化酶亚基1（COI）的DNA为基础的识别系统，可以帮助解决这种多样性。虽然过去的工作已经验证了COI序列诊断某些分类组中物种的能力，但本研究将这些分析扩展到整个动物王国。结果表明，COI的序列分歧定期使在所有动物门的近缘物种的歧视，除了刺胞动物。物种诊断的成功反映了大多数动物群体中COI的高序列变化率和种内线粒体DNA分歧的限制，至少部分是通过与核基因组相互作用介导的选择性扫描而产生的。
With millions of species and their life-stage transformations, the animal kingdom provides a challenging target for taxonomy. Recent work has suggested that a DNA-based identification system, founded on the mitochondrial gene, cytochrome c oxidase subunit 1 (COI), can aid the resolution of this diversity. While past work has validated the ability of COI sequences to diagnose species in certain taxonomic groups, the present study extends these analyses across the animal kingdom. The results indicate that sequence divergences at COI regularly enable the discrimination of closely allied species in all animal phyla except the Cnidaria. This success in species diagnosis reflects both the high rates of sequence change at COI in most animal groups and constraints on intraspecific mitochondrial DNA divergence arising, at least in part, through selective sweeps mediated via interactions with the nuclear genome.