Smartphone-based chemiluminescence detection of aflatoxin B1 via labelled and label-free dual sensing systems.
Smartphone-based chemiluminescence detection of aflatoxin B1 via labelled and label-free dual sensing systems.
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DOI：
10.1016/j.foodchem.2023.135654
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发表时间：
2023-02
期刊：
影响因子：
8.8
通讯作者：
Junning Ma;Y. Guan;F. Xing;Yan Wang;Xu Li;Qiang Yu;Xiao-Bo Yu
中科院分区：
文献类型：
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作者：
Junning Ma;Y. Guan;F. Xing;Yan Wang;Xu Li;Qiang Yu;Xiao-Bo Yu
为了开发用于食品中黄曲霉毒素B1现场检测的传感平台，我们开发了基于智能手机的黄曲霉毒素B1化学发光检测，通过标记和无标记双模式进行检测。标记模式的特征是双链霉亲和素-生物素介导的信号放大，在1-100 ng/mL的线性范围内获得0.04 ng/mL的检测限（LOD）。为了减少标记系统的复杂性，构建了基于裂解适体和裂解DNA酶的无标记模式。在1-100 ng/mL的线性范围内，生成了令人满意的LOD（0.33 ng/mL）。标记和无标记传感系统在AFB 1加标的玉米和花生仁样品中均获得了优异的回收率。最后，基于定制组件和Android应用程序，将两套系统成功集成到基于智能手机的便携式设备中，实现了与商用酶标仪相当的AFB 1检测能力。我们的系统在食品供应链中的AFB 1现场检测方面具有巨大的潜力。
To develop a sensing platform for onsite determination of AFB1in foodstuffs, we developed smartphone-based chemiluminescence detection of AFB1via labelled and label-free dual modes. The labelled mode was characteristic of double streptavidin–biotin mediated signal amplification, obtaining limit of detection (LOD) of 0.04 ng/mL in the linear range of 1–100 ng/mL. To reduce the complexity in the labelled system, a label-free mode based on both split aptamer and split DNAzyme was fabricated. A satisfactory LOD of 0.33 ng/mL was generated in the linear range of 1–100 ng/mL. Both labelled and label-free sensing systems achieved outstanding recovery rate in AFB1-spiked maize and peanut kernel samples. Finally, two systems were successfully integrated into smartphone-based portable device based on custom-made components and android application, achieving comparable AFB1detection ability to a commercial microplate reader. Our systems hold huge potential for AFB1onsite detection in food supply chain.