SYNTHESIS OF PHAGE-SPECIFIC ALPHA- AND BETA-GLUCOSYL TRANSFERASES DIRECTED BY T-EVEN DNA IN VITRO
SYNTHESIS OF PHAGE-SPECIFIC ALPHA- AND BETA-GLUCOSYL TRANSFERASES DIRECTED BY T-EVEN DNA IN VITRO
复制标题
DOI：
10.1073/pnas.62.3.892
复制
发表时间：
1969-01-01
影响因子：
11.1
通讯作者：
SCHWEIGER, M
中科院分区：
文献类型：
--
作者：
GOLD, LM;SCHWEIGER, M
-噬菌体T4DNA，当加入来自未受感染的大肠杆菌的核糖核酸和蛋白质合成系统时，引导病毒特异性葡萄糖转移酶的体外合成。T4特异性的α-和β-葡萄糖转移酶在感染后早期在体内合成，并作用于糖基化噬菌体DNA的羟甲基胞嘧啶残基。葡萄糖基转移酶的合成依赖于T4DNA的转录，也依赖于蛋白质的合成。来自T4突变体的DNA不能诱导葡萄糖基转移酶，但在体外未能诱导酶的形成，尽管蛋白质合成没有受到影响。
—Bacteriophage T4 DNA, when added to a ribonucleic acid- and protein-synthesizing system from uninfectedEscherichia coli, directed thein vitrosynthesis of virus-specific glucosyl transferases.The T4-specific α- and β-glucosyl transferases are synthesizedin vivoearly after infection, and function to glucosylate the hydroxymethylcytosine residues of phage DNA. Thein vitroglucosyl transferase synthesis was dependent upon transcription of T4 DNA, as well as upon protein synthesis. DNA from T4 mutants unable to induce glucosyl transerases failed to induce enzyme formationin vitro, although protein synthesis was unimpaired.