Serum IgE antibodies bind to the epidermal side of the basement membrane zone splits in bullous pemphigoid
Serum IgE antibodies bind to the epidermal side of the basement membrane zone splits in bullous pemphigoid
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血清 IgE 抗体与大疱性类天疱疮基底膜区分裂的表皮侧结合
DOI：
10.1111/j.1365-2133.1992.tb15131.x
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发表时间：
1992
影响因子：
10.3
通讯作者：
A. Rebora
中科院分区：
文献类型：
--
作者：
A. Parodi;A. Rebora
先生.大多数大疱性类天疱疮（BP）患者血清IgR水平较高，这似乎与疾病活动有关。它们所针对的皮肤结构是未知的。为了探讨IgK抗体在BP中的可能作用。我们研究了9例具有典型临床和组织病理学特征的BP患者（7例男性，2例女性）。他们都没有过敏史，也没有接受免疫抑制治疗。用放射免疫测定法（RIA）测定了三名具有高血清IgK水平（587- 43 μ l/nil;正常值< 100）的特应性患者和两名具有低血清IgE水平的受试者作为对照。通过标准方法使用Meloy GAM IgF FITC和Kallestad GAII IgC，伊加进行直接和间接免疫荧光（IF）。IgM.C3. C4 FITC。人皮肤，完整，并在ludda板水平用1 M NaCl裂开。和猴食管，作为间接IF的底物。采用放射免疫法测定血清IgE水平。在直接IF中，所有患者均在基底膜区（HM 7）以BP或获得性大疱性表皮炎（F.BA）典型的线性模式存在IgG和C3沉积。两名患者在BMZ处也有非常轻微的不连续IgK沉积。在I 'atieiU 4中，沉积物是粒状的，而在患者T中是线性的。6例患者血清中存在针对BMZ的IgG抗体。用猴子的食道作为基质。在5名患者中，抗体与IM NaCl诱导的分裂的顶部结合（图1），在1名患者中，抗体与顶部和底部结合。抗BM 7 IgG的滴度范围为1/40至1/40。7例患者血清IgM水平大于1（10 U/ml）（150 ± 112 3U/ml），与疾病的严重程度无关。所有对照组均无抗BMZ的循环抗体。抗RMZ IgF。使用猴食管作为底物，在6名患者中检测到抗体，滴度范围在1/40和1/160之间。在所有病例中，它们都能到达人类皮肤破裂处的大疱顶部（图2）。使用猴食管作为底物，所有对照均不具有抗RMZ [gi]。所有具有α-BMZ IgG的患者也具有抗BMZ IgE。即使是在I 'igure 2.间接IF锡I » NaCl人分裂皮肤，lyE到屋顶具有线性图案。hind
SIR. Most patients with bullous pemphigoid (BP) have high serum levels of IgR' '' which appear to be related to disease activity. The skin structure at which they are directed is unknown. In order to investigate the possible role of IgK antibodies in BP. we studied nine patients (seven male, two female) with typical clinical and histopathological features of BP. None had a history of atopy, nor were they taking immunosuppressive therapy. Three atopic patients with high serum IgK levels (587-43f) ll/nil; normal value < 100) as determined by radioimmunoassay (RIA) and two subjects with low serum IgE levels served as controls. Direct and indirect immunofluorescence (IF) were performed by standard methods using Meloy GAM IgF FITC and Kallestad GAII IgC, IgA. IgM.C3. C4 FITC. Human skin, both entire, and split at tbe lamina ludda level with 1 M NaCI. and monkey oesophagus, served as substrates for indirect IF. The serum IgE levels were determined by RIA. On direct IF all patients had deposits of IgG and C3 at the basement membrane zone (HM7) in a linear pattern typical of BP or epidermolysis bullosa acquisita (F.BA). Two patients also had very slight discontinuous IgK deposits at the BMZ. In I'atieiU 4 the deposits were granidar and in Patient T linear. Six patients had IgG antibodies in their serum directed at the BMZ. using monkey oesophagus as a substrate. The antibodies bound to the roof of I M NaCl-induced splits in five patients (Fig. 1) and to both the roof and the base in one. The titres of anti-BM7 IgG ranged from 1/40 lo l/(i40. Seven patients had •serum IgM levels grt-att-r than 1 (10 U/ml (1 50112 3 U/ml) that were apparently unrelated to the extent of the disease. None of ihe controls had circulating antib{Kiies to BMZ. Anti-RMZ IgF. antibodies were detected in six patients using monkey oesophagus as substrate, with titres ranging between 1/40 and 1/160. They hound to the roof of the bulla in human split skin in ail cases (Fig. 2). None of the controls had anti-RMZ [gi; using monkey oesophagus as a substrate. All of the patients with an(i-BMZ IgG also had anti-BMZ IgE. even when the I'igure 2. indirecl IF tin I » NaCI human split skin, lyE to the roof with a linear pattern. hind