Protection of rabbit kidney from ischemia/reperfusion injury by green tea Polyphenol pretreatment
Protection of rabbit kidney from ischemia/reperfusion injury by green tea Polyphenol pretreatment
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DOI：
10.1007/bf02977370
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发表时间：
2007-11-01
影响因子：
6.7
通讯作者：
Park, Jong-Chul
中科院分区：
文献类型：
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作者：
Rah, Dong Kyun;Han, Dong-Wook;Park, Jong-Chul
活性氧（Reactive oxygen species，ROS）参与了缺血/再灌注（ischemia/reperfusion，I/R）后肾损伤的发病机制。近年来，人们发现绿色茶多酚（GTP）具有保护心肌和肝脏免受I/R损伤的作用。然而，很少有人注意到GTP对肾脏的保护作用。本研究旨在确定GTP是否可以保护肾细胞免受缺血损伤。将兔分为三组：对照组（假手术组）、I/R +赋形剂组（生理盐水组）和I/R + GTP组。每组由6只兔子组成。动物分别接受30、60、90和120 min的缺血，随后再灌注24 h。在I/R开始前45分钟给予GTP（200 μ g/kg）或溶媒。结果表明，在缺血90分钟后，GTP给药导致肾损伤显著减轻（P < 0.05），如血清中肌酐和尿素氮水平降低所示。组织学检查结果表明，GTP预处理可抑制I/R引起的近端小管坏死和脱落。检查还显示I/R损伤兔髓质坏死面积减少，肾小球塌陷减少。此外，在GTP处理的肾脏中，CD 8（+）T细胞的浸润显著减少。本研究结果提示，GTP可通过抑制I/R依赖的氧化应激而减轻肾损伤，可作为抗氧化剂用于肾移植。
Reactive oxygen species (ROS) have been implicated in the pathogenesis of renal injury after ischemia/reperfusion (I/R). Recently, green tea polyphenols (GTP) have been found to protect the myocardium and liver against l/R injury. Less attention, however, has been paid to the protective effects of GTP with respect to the kidneys. This study was designed to determine whether GTP could protect renal cells from ischemic injury. The rabbits were divided into three groups of equal size: control (sham-operated), l/R + vehicle (normal saline) and l/R + GTP groups. Each group consisted of six rabbits. Animals underwent 30, 60, 90 and 120 min of ischemia, followed by 24 h of reperfusion, respectively. GTP (200 mu g/kg) or the vehicle was administered 45 min prior to commencement of I/R. The results demonstrated that GTP administration resulted in a significant (P < 0.05) reduction of renal damage after 90 min of ischemia, as indicated by the decreased levels of creatinine and urea nitrogen in serum. These results were confirmed by histological examinations, which showed that GTP pretreatment inhibited necrosis and sloughing of the proximal tubules induced by I/R. Examinations also showed decreased necrotic areas in the medulla and decreased glomerular collapse in the I/R-injured rabbits. Moreover, the infiltration of CD8(+) T cells was considerably decreased in GTP-treated kidneys. The results of this study,suggest that GTP can reduce renal injury by preventing the oxidative stress dependent on l/R and may be used in renal transplantation as an antioxidant.