Sialylated glycan-modulated biomimetic ion nanochannels driven by carbohydrate-carbohydrate interactions
Sialylated glycan-modulated biomimetic ion nanochannels driven by carbohydrate-carbohydrate interactions
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DOI：
10.1038/s41427-022-00399-z
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发表时间：
2022-12-01
影响因子：
9.7
通讯作者：
Qing，Guangyan
中科院分区：
文献类型：
--
作者：
Xiao，Jie;Lu，Wenqi;Qing，Guangyan
离子通道在调节细胞内外离子环境中起着关键作用。电压门控离子通道（vgic）末端的唾液化聚糖（Sialylated glycans, SGs）丰富，直接控制vgic的开关，而细胞表面的SGs也与病毒感染、肿瘤生长和转移密切相关。在这里，我们报道了一种仿生离子纳米通道装置，可以由SG精确调节。该纳米通道器件由具有锥形纳米通道的化学蚀刻聚对苯二甲酸乙二醇酯薄膜和聚乙烯亚胺-g- malcopyrano苷（缩写为Mal-PEI）组成。麦芽糖是Mal-PEI中的核心结合单元，与SG形成多个氢键相互作用，触发聚合物链的球状到线圈转变，阻碍跨膜离子运输，导致纳米通道的离子电流显著降低。根据离子电流的变化，该装置可以精确区分α2-3-和α2-6链的唾液基乳糖，以及SGs和中性糖。重要的是，该纳米通道装置可以实时监测α2,6-唾液基转移酶催化的乳糖唾液化过程，在酶活性测定和体外酶鉴定方面具有良好的潜力。本研究构建了一个具有选择性和智能离子门控行为的SG调制纳米通道，在SG响应性和酶活性监测方面表现出独特的优势。
Ion channels play key roles in regulating the ion environment inside and outside the cell. Sialylated glycans (SGs) at the terminus of voltage-gated ion channels (VGICs) are abundant and directly control the switch of VGICs, while SGs on the cell surface are also closely related to virus infection, tumor growth, and metastasis. Here, we report a biomimetic ion nanochannel device that can be precisely regulated by SG. The nanochannel device is composed of a chemically etched polyethylene terephthalate film featuring conical nanochannels and a polyethyleneimine-g-malcopyranoside (abbreviated to Mal-PEI). Maltose, core-binding units in Mal-PEI, forms multiple hydrogen-bonding interactions with SG, which triggers globule-to-coil transition of the polymer chain and blocks transmembrane ion transport, resulting in a remarkable decrease in the ionic current of the nanochannel. Based on the changes in the ionic current, this device can precisely discriminate α2-3- and α2-6-linked sialyllactose, as well as SGs and neutral saccharides. Importantly, the nanochannel device can monitor the sialylation process of lactose catalyzed by α2,6-sialyltransferase in real time, showing its good potential in enzyme activity determination and in vitro enzyme identification. This work constructs an SG-modulated nanochannel with selective and smart ion-gating behavior, exhibiting unique advantages in SG responsiveness and enzymatic activity monitoring.