A LONG AND COMPLEX ENHANCER ACTIVATES TRANSCRIPTION OF THE GENE CODING FOR THE HIGHLY ABUNDANT IMMEDIATE EARLY MESSENGER-RNA IN MURINE CYTOMEGALOVIRUS
A LONG AND COMPLEX ENHANCER ACTIVATES TRANSCRIPTION OF THE GENE CODING FOR THE HIGHLY ABUNDANT IMMEDIATE EARLY MESSENGER-RNA IN MURINE CYTOMEGALOVIRUS
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DOI：
10.1073/pnas.82.24.8325
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发表时间：
1985-12-01
影响因子：
11.1
通讯作者：
KOSZINOWSKI, UH
中科院分区：
文献类型：
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作者：
DORSCHHASLER, K;KEIL, GM;KOSZINOWSKI, UH
使用猿猴病毒40“增强子陷阱”方法，我们鉴定了位于鼠巨细胞病毒主要立即早期基因上游的转录增强子。该增强剂具有几个显着的特性。 (i)与人巨细胞病毒的增强子一起，是迄今为止发现的最强的转录增强子。 (ii) 它是一个极长的增强子，跨越 > 700 个碱基对。 (iii) 它由相当复杂的序列重复模式组成，其中最长的是 181 个碱基对。此外，几种类型的短序列基序以单体、异二聚体或同二聚体（回文）形式分散在整个增强子中。这些基序已被鉴定为其他增强子和启动子的组成部分，并且它们可能是特定核因子的结合位点。我们的分析表明增强子由短保守序列基序的模块化排列组成，并且增强子强度与这些基序的冗余度相关。
Using the simian virus 40 "enhancer trap" approach, we have identified a transcription enhancer located just upstream of the major immediate early gene of murine cytomegalovirus. This enhancer has several striking properties. (i) Together with the enhancer of human cytomegalovirus, it is the strongest transcription enhancer found to date. (ii) It is an extremely long enhancer, spanning > 700 base pairs. (iii) It consists of a rather complex pattern of sequence repeats, the longest of which is 181 base pairs. Also, several types of short sequence motifs are scattered throughout the enhancer in monomeric, heterodimeric, or homodimeric (palindromic) form. These motifs have been identified to be components of other enhancers and promoters, and they are presumably binding sites for specific nuclear factors. Our analysis suggests that enhancers are composed of a modular arrangement of short conserved sequence motifs and that enhancer strength is correlated with the redundancy of these motifs.