The presence of microcystins and other cyanobacterial bioactive peptides in aquatic fauna collected from Greek freshwaters
The presence of microcystins and other cyanobacterial bioactive peptides in aquatic fauna collected from Greek freshwaters
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DOI：
10.1016/j.aquatox.2006.02.001
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发表时间：
2006-06-10
影响因子：
4.5
通讯作者：
Sivonen, K.
中科院分区：
文献类型：
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作者：
Gkelis, S.;Lanaras, T.;Sivonen, K.
有毒的水华蓝藻可导致动物死亡，并对人类健康造成不利影响。水华可能含有微囊藻毒素(MC)、蓝藻七肽肝毒素和其他多肽，如鱼腥藻多肽和鱼腥藻多肽。MCS已被证明存在于各种水生生物中，包括贻贝、水蜗牛、甲壳类和鱼类。8种鱼类(Acipenser gueldenstaedtii、Carassius auratus、Carassius gibelo、Cyprinus Carpio、Perca River viatilis、Rutilus rubilio、Silurus aristotelis和Silurus glanis)、青蛙(Rana Eperotica)、贻贝(Anodonta sp.)采用高效液相色谱(HPLC)、蛋白磷酸酶1(PP1)抑制法(PP1IA)和酶联免疫吸附试验(EL ISA)对水螺(Viviparus Conectus)进行了检测。用PP1IA和ELISA法检测所有鱼类、青蛙、贻贝和水螺样品中均检出MC(S)，其中包括青蛙和淡水蜗牛V Contectus，这些样品中MCs的存在尚不清楚。鱼和蛙肌肉和内脏组织中MC含量分别为20~1500 ng g(-1)dw和25~5400 ng g(-1)dw。贻贝和水螺组织中MC浓度范围为1650~3495 ng g(-1)dw。高效液相色谱分析显示，峰具有与鱼腥草素或鱼腥藻内酯类化合物相同的紫外光谱，这是以前在水生动物组织中未曾见过的。检测到的化合物浓度在1.5-230微克克(-1)干重之间。PP1IA法与ELISA法的比较表明，PP1IA法的测定值高于ELISA法。动物组织中的鱼腥草素和/或鱼腥草素可能解释了PP1IA值较高的原因，因为我们发现纯化的鱼腥草素A(抑制率为45-60%)和B(抑制率为5-75%)对PP1活性有抑制作用。纯化的鱼腥藻90A和90B对PP1的抑制活性较弱，分别为5-35%和5-23%。这是首次在南欧淡水采集的水生动物中发现MC的报道。(C)2006爱思唯尔B.V.保留所有权利。
Toxic bloom-forming cyanobacteria can cause animal death and adversely affect human health. Blooms may contain microcystins (MCs), cyanobacterial heptapeptide hepatotoxins and other peptides such as anabaenopeptins and anabaenopeptilides. MCs have been shown to occur in various aquatic organisms including mussels, water snails, crustaceans and fish. Muscle and viscera samples from eight species of fish (Acipenser gueldenstaedtii, Carassius auratus, Carassius gibelio, Cyprinus carpio, Perca fluviatilis, Rutilus rubilio, Silurus aristotelis and Silurus glanis), a frog (Rana eperotica), a mussel (Anodonta sp.) and a water snail (Viviparus contectus) were analyzed by high-performance liquid chromatography (HPLC), protein phosphatase 1 (PP1) inhibition assay (PP1IA) and ELISA. MC(s) was detected in all fish, frog, mussel and water snail samples tested by PP1IA and ELISA, including the frog R. eperotica and the freshwater snail V contectus, in which the occurrence of MCs was not previously known. MC concentration ranged from 20 to 1500 ng g(-1) dw and from 25 to 5400 ng g(-1) dw in muscle and visceral tissue of fishes and frogs, respectively. In mussel and water snail tissue MC concentration ranged from 1650 to 3495 ng g(-1) dw. HPLC analysis revealed peaks having the same UV spectrum as anabaenopeptin- or anabaenopeptilide-like compounds, not previously known to occur in aquatic fauna tissue. The concentrations of the compounds detected ranged from 1.5 to 230 mu g g(-1) dw. Comparison of the PP1IA and ELISA showed that values obtained with PP1IA where higher than those obtained with ELISA. Anabaenopeptins and/or anabaenopeptilides occurring in faunal tissue may account for the higher PP1IA values as we found that PP1 activity was inhibited by the purified anabaenopeptins A (45-60% inhibition) and B (5-75% inhibition). Purified anabaenopeptilides 90A and 90B exhibited weaker PP1 inhibition activity (5-35 and 5-23% inhibition, respectively). This is the first report of MC occurrence in aquatic animals collected from freshwaters of southern Europe. (c) 2006 Elsevier B.V. All rights reserved.