The applications of universal DNA base analogues
The applications of universal DNA base analogues
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DOI：
10.1093/nar/29.12.2437
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发表时间：
2001-06-15
影响因子：
14.9
通讯作者：
Loakes, D
中科院分区：
文献类型：
--
作者：
Loakes, D
通用碱基类似物与每个天然DNA/RNA碱基形成“碱基对”，它们之间几乎没有区别。许多这样的类似物已被制备和它们的应用作为生化工具的调查。这些类似物中的大多数是非氢键的、疏水的、芳香族的“碱基”，其通过堆叠相互作用来稳定双链体DNA。本文综述了通用碱的文献（至2000年），详细介绍了研究的类似物，并讨论了它们的用途和局限性。
A universal base analogue forms 'base pairs' with each of the natural DNA/RNA bases with little discrimination between them. A number of such analogues have been prepared and their applications as biochemical tools investigated. Most of these analogues are non-hydrogen bonding, hydrophobic, aromatic 'bases' which stabilise duplex DNA by stacking interactions. This review of the literature of universal bases (to 2000) details the analogues investigated, and their uses and limitations are discussed.