Sequence-Specific Polyampholyte Phase Separation in Membraneless Organelles
Sequence-Specific Polyampholyte Phase Separation in Membraneless Organelles
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DOI：
10.1103/physrevlett.117.178101
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发表时间：
2016-10-17
影响因子：
8.6
通讯作者：
Chan, Hue Sun
中科院分区：
文献类型：
--
作者：
Lin, Yi-Hsuan;Forman-Kay, Julie D.;Chan, Hue Sun
富含电荷和/或芳香族的固有无序蛋白质（IDP）的液-液相分离在无膜细胞器的生物功能中是至关重要的。这一最新发现的许多物理学原理仍有待阐明。在这里，我们提出了一个理论，在随机相位近似占聚两性电解质相分离的静电效应，产生的预测与最近的实验IDP Ddx 4一致。该理论适用于任何电荷模式，从而为研究IDP相分离的序列依赖性提供了一个通用的分析框架。
Liquid-liquid phase separation of charge- and/or aromatic-enriched intrinsically disordered proteins (IDPs) is critical in the biological function of membraneless organelles. Much of the physics of this recent discovery remains to be elucidated. Here, we present a theory in the random phase approximation to account for electrostatic effects in polyampholyte phase separations, yielding predictions consistent with recent experiments on the IDP Ddx4. The theory is applicable to any charge pattern and thus provides a general analytical framework for studying sequence dependence of IDP phase separation.