Interference reflection microscopy.
Interference reflection microscopy.
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DOI：
10.1002/0471143030.cb0423s45
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发表时间：
2009-12
影响因子：
--
通讯作者：
Bunnell, Stephen C
中科院分区：
文献类型：
--
作者：
Barr, Valarie A;Bunnell, Stephen C
干涉反射显微镜是一种用于研究细胞在盖玻片上的粘附或细胞迁移的光学技术。反射光波的干涉产生具有高对比度和清晰度的图像。荧光显微镜可以用来检查几乎任何一种停留在玻璃表面上的细胞，尽管它在检查细胞和基质之间紧密接触的部位时最有用。本章介绍了获得细胞的显微图像的方法，特别强调了激光扫描共聚焦显微镜（LSCM）的显微成像，因为大多数LSCM已经能够记录这些图像，而无需对仪器进行任何修改。提出了用于成像固定和活细胞以及同时多通道捕获荧光和反射图像的技术。
Interference Reflection microscopy is an optical technique used to study cell adhesion or cell mobility on a glass coverslip. The interference of reflected light waves generates images with high contrast and definition. IRM can be used to examine almost any cell that will rest upon a glass surface, although it is most useful in examining sites of close contact between a cell and substratum. This chapter presents methods for obtaining IRM images of cells with particular emphasis on IRM imaging with a laser scanning confocal microscope (LSCM), as most LSCM are already capable of recording these images without any modification of the instrument. Techniques are presented for imaging fixed and live cells as well as simultaneous multi-channel capture of fluorescence and reflection images.