Synthesis, characterization, DNA-binding and photocleavage of complexes [Ru(phen)2(6-OH-dppz)]2+ and [Ru(phen)2(6-NO2-dppz)]2+
Synthesis, characterization, DNA-binding and photocleavage of complexes [Ru(phen)2(6-OH-dppz)]2+ and [Ru(phen)2(6-NO2-dppz)]2+
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DOI：
10.1016/j.jinorgbio.2005.09.004
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发表时间：
2005-12-01
影响因子：
3.9
通讯作者：
Ji, LN
中科院分区：
文献类型：
--
作者：
Liu, XW;Li, J;Ji, LN
两个新的建筑群。合成了([Ru(phen)(2)(6-OH-d ppz)](2+))(1)和([Ru(phen)(2)(6-NO2-dppz)](2+)) (2)（phen = 1,10-菲罗啉；6-OH-dppz = 6-羟基-二吡啶[3,2-a:2',3'-c]吩那嗪；6-NO2-dppz = 6-硝基-二吡啶[3.2-a:2',3'-c]吩那嗪），并通过元素分析、ES-MS（电喷雾质谱）、H-1 NMR、UV-Vis（紫外可见）和CV（循环伏安法）对其进行了表征。通过光谱方法和粘度测量研究了这两种配合物的dna结合行为。结果表明，两种复合物均以插层方式与小牛胸腺DNA （CT-DNA）结合，且复合物2的DNA结合亲和力大于复合物1。此外，复合物1能促进pBR322 DNA在照射下的光裂解，而复合物2在照射和黑暗中都能促进pBR322 DNA的裂解，且照射后的裂解效率更高。用密度泛函理论（DFT）方法对这些配合物进行了理论研究。这两种配合物的dna结合行为的差异可以通过DFT计算得到合理的解释。(c) 2005爱思唯尔公司版权所有。
Two new complexes. ([Ru(phen)(2)(6-OH-d ppz)](2+)) (1) and ([Ru(phen)(2)(6-NO2-dppz)](2+)) (2) (phen = 1,10-phenanthroline; 6-OH-dppz = 6-hydroxyl-dipyrido[3,2-a:2',3'-c]phenazine; 6-NO2-dppz = 6-nitro-dipyrido[3.2-a:2',3'-c]phenazine), have been synthesized and characterized by elemental analysis, ES-MS (electrospray mass spectra), H-1 NMR, UV-Vis (UV-visible) and CV (cyclic voltammetry). The DNA-binding behaviors of both complexes have been studied by spectroscopic methods and viscosity measurements. The results indicate that the two complexes all bind to calf thymus DNA (CT-DNA) in an intercalative mode, and the DNA-binding affinity of complex 2 is greater than that of complex 1. In addition, complex 1 can promote photocleavage of pBR322 DNA upon irradiation, whereas complex 2 can promote cleavage of pBR322 DNA both upon irradiation and in the dark, with more efficient cleavage occurring upon irradiation. Theoretical studies for these complexes have been also carried out with the density functional theory (DFT) method. The difference in the DNA-binding behaviors of the two complexes can be reasonably explained by the DFT calculations. (c) 2005 Elsevier Inc. All rights reserved.