Relationships between aerobic and anaerobic energy production in turtle brain in situ.
Relationships between aerobic and anaerobic energy production in turtle brain in situ.
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龟脑原位有氧和无氧能量产生之间的关系。
DOI：
10.1152/ajpregu.1984.247.4.r740
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Sick,TJ
中科院分区：
文献类型：
--
作者：
Lutz,PL;McMahon,P;Rosenthal,M;Sick,TJ
为了确定海龟大脑线粒体活动与高能中间体之间的关系，根据细胞色素 a、a3 的氧化还原状态，有时将大脑原位冷冻。脑乳酸的增加表明，在缺氧期间氧化反应仍在进行时，发生了向厌氧的转变，并且厌氧的信号并不是线粒体电子传递的完全失败。磷酸肌酸 (CrP)、ATP、ADP 和 AMP 的减少表明缺氧期间它们的消耗超过了产生。在接下来的 120 分钟缺氧期间，CrP 进一步下降，但 ATP、ADP 和 AMP 与对照值相比没有显着变化。这些数据表明 ATP 对限速关键糖酵解酶（如磷酸果糖激酶）没有抑制作用。计算表明缺氧期间 ATP 产量下降。这项研究表明，与哺乳动物大脑相比，CrP 值降低、ATP 产生减少和乳酸水平升高并不妨碍乌龟大脑的离子稳态和电活动，并且在缺氧期间该大脑可以免受乳酸或 pH 值的损害。
To define the relationships between brain mitochondrial activity and high-energy intermediates in the turtle, brains were frozen in situ at times determined by the reduction-oxidation status of cytochrome a,a3. Increases in brain lactate indicated that transition to anaerobiosis occurred while oxidative reactions were still ongoing during hypoxia and that the signal for anaerobiosis is not the complete failure of mitochondrial electron transfer. Decreases in creatine phosphate (CrP), ATP, ADP, and AMP demonstrate that their consumption exceeded production during hypoxia. CrP decreased further during the following 120 min of anoxia, but ATP, ADP, and AMP were not significantly altered from control values.These data suggest that ATP is not inhibitory to rate-limiting, key glycolytic enzymes such as phosphofructokinase. Calculations indicate that ATP production declined during anoxia. This study indicates that decreased CrP values, decreased ATP production, and high lactate levels do not preclude ion homeostasis and electrical activity of turtle brain and that this brain is protected against lactate or pH damage during anoxia, in contrast to mammalian brain.