ANNEXIN1 mediates calcium-dependent systemic defense in Arabidopsis plants upon herbivory and wounding
ANNEXIN1 mediates calcium-dependent systemic defense in Arabidopsis plants upon herbivory and wounding
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DOI：
10.1111/nph.17277
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发表时间：
2021-03-19
期刊：
影响因子：
9.4
通讯作者：
Mithoefer, Axel
中科院分区：
文献类型：
--
作者：
Malabarba, Jaiana;Meents, Anja K.;Mithoefer, Axel
细胞钙（Ca）瞬态信号是一种内源性信号，参与局部和全身的信号传递以及对环境胁迫的防御激活，包括伤害和草食。然而，并不是所有参与信号传导的Ca2+通道都被确定，它们的作用模式也不完全清楚。植物膜联蛋白是一种能够与阴离子磷脂结合的蛋白质，可以表现出类似钙通道的活性。拟南芥ANNEXIN1 （ANN1）可能参与钙的转运。在这里，我们报道了损伤和模拟草食诱导的细胞质游离钙升高在ann1功能丧失植物的全身叶片中受损。我们为ANN1在被草食性沿海夜蛾（Spodoptera littoralis）幼虫攻击的植物的局部和系统防御中的作用提供了证据。生物测定鉴定出ANN1为阳性防御调节因子。以ann1为食的沿海夜蛾的增重显著高于以野生型为食的夜蛾，而以ann1过表达系为食的夜蛾的增重明显低于以野生型为食的夜蛾。草食处理和伤害处理均诱导了茉莉酸盐积累和防御基因表达等防御相关反应。这些反应只存在于局部，在ann1植物的系统叶片中被强烈减弱。结果表明，在草食性昆虫攻击的植物中，ANN1在激活系统防御而非局部防御中起重要作用。
Cellular calcium (Ca) transients are endogenous signals involved in local and systemic signaling and defense activation upon environmental stress, including wounding and herbivory. Still, not all Ca2+ channels contributing to the signaling have been identified, nor are their modes of action fully known. Plant annexins are proteins capable of binding to anionic phospholipids and can exhibit Ca channel-like activity. Arabidopsis ANNEXIN1 (ANN1) is suggested to contribute to Ca transport.Here, we report that wounding and simulated-herbivory-induced cytosolic free Ca elevation was impaired in systemic leaves in ann1 loss-of-function plants. We provide evidence for a role of ANN1 in local and systemic defense of plants attacked by herbivorous Spodoptera littoralis larvae.Bioassays identified ANN1 as a positive defense regulator. Spodoptera littoralis feeding on ann1 gained significantly more weight than larvae feeding on wild-type, whereas those feeding on ANN1-overexpressing lines gained less weight. Herbivory and wounding both induced defense-related responses on treated leaves, such as jasmonate accumulation and defense gene expression. These responses remained local and were strongly reduced in systemic leaves in ann1 plants.Our results indicate that ANN1 plays an important role in activation of systemic rather than local defense in plants attacked by herbivorous insects.