Cellular prion protein released on exosomes from macrophages binds to Hsp70.
Cellular prion protein released on exosomes from macrophages binds to Hsp70.
复制标题
巨噬细胞外泌体释放的细胞朊病毒蛋白与 Hsp70 结合。
DOI：
10.1093/abbs/gmq028
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发表时间：
2010-05
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
作者：
Pron病是一种传染性和致命性的神经退行性疾病。细胞内的PrP(C)转化为PrP(SC)的错误折叠异构体，与PrP(SC)的感染有关。免疫系统在促进病毒感染从外周向中枢神经系统扩散的过程中起着重要作用。巨噬细胞被认为与PrP(SC)的运输和复制有关。因此，了解PrP(C)在巨噬细胞中的转运对于探索PrP(SC)的转运机制具有重要意义。在这里，我们从Ana-1巨噬细胞系的培养液中分离出外切体，并用免疫印迹、免疫电子显微镜和免疫共沉淀法研究了外切体转运的PrP(C)以及分泌型外切体中PrP(C)与Hsp70的相互作用。结果表明，从巨噬细胞培养上清液中分离出的囊泡具有胞外体的特征，并带有PrP(C)。PrP(C)在胞内环境和分泌外体中均与Hsp70结合。PrP(C)与外体标记蛋白Tag101和Flotillin-1无相互作用。这些结果表明，PrP(C)可能通过巨噬细胞分泌的外切体外化，而Hsp70可能在PrP(C)通过分泌性外切体释放的过程中发挥作用。
Prion diseases are infectious and fatal neurodegenerative disorders. The cellular prion protein (PrP(C)) converting into misfolded isoform of prion protein (PrP(Sc)) is responsible for prion disease infection. Immune system plays an important role in facilitating the spread of prion infections from the periphery to the central nervous system. Macrophages were considered associated with the transportation and replication of PrP(Sc). So, understanding the PrP(C) trafficking in macrophages is important to explore the transport mechanism for PrP(Sc). Here, we isolated exosomes from the culture medium of Ana-1 macrophage cell line and investigated the PrP(C) trafficked by exosomes and the interaction of PrP(C) with Hsp70 in secreted exosomes by western blotting, immunoelectron microscopy, and co-immunoprecipitation. The results showed that the isolated vesicles from the culture medium of macrophages were characterized by exosomes and bore PrP(C). And PrP(C) bound to Hsp70 both in intracellular environment and secreted exosomes. In contrast, PrP(C) had no interaction with marker proteins of exosomes, Tag101 and Flotillin-1. These results suggested that PrP(C) present in extracellular space might be externalized through secreted exosomes from macrophages, and Hsp70 may play roles in the process of PrP(C) released via secreted exosomes.
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影响因子：
2.3
作者：
Porto-Carreiro, I;Février, B;Raposo, G
通讯作者：
Raposo, G
DOI：
10.1073/pnas.152330499
发表时间：
2002-12-10
影响因子：
11.1
作者：
Bonini, NM
通讯作者：
Bonini, NM
影响因子：
4.8
作者：
Wubbolts, R;Leckie, RS;Stoorvogel, W
通讯作者：
Stoorvogel, W
DOI：
10.1002/1096-9896(200007)191:3
发表时间：
2000-07
期刊：
The Journal of Pathology
影响因子：
--
作者：
M. Jeffrey;G. McGovern;C. Goodsir;Karen L Brown;M. Bruce
通讯作者：
M. Jeffrey;G. McGovern;C. Goodsir;Karen L Brown;M. Bruce
DOI：
10.1083/jcb.101.3.942
发表时间：
1985-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Pan BT;Teng K;Wu C;Adam M;Johnstone RM
通讯作者：
Johnstone RM