Detection of Merkel Cell Polyomavirus in Merkel Cell Carcinoma and Kaposi's Sarcoma
Detection of Merkel Cell Polyomavirus in Merkel Cell Carcinoma and Kaposi's Sarcoma
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DOI：
10.1002/jmv.21608
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发表时间：
2009-11-01
影响因子：
12.7
通讯作者：
Sata, Tetsutaro
中科院分区：
文献类型：
--
作者：
Katano, Harutaka;Ito, Hideki;Sata, Tetsutaro
默克尔细胞癌是一种罕见的恶性肿瘤，有时发生在老年人的皮肤。最近，在默克尔细胞癌中鉴定出一种新的人类多瘤病毒，默克尔细胞多瘤病毒（MCPyV）。在本研究中，MCPyV-DNA检测11例（55%）默克尔细胞癌中的6例（巢式PCR和实时PCR）。组织学上，MCPyV阳性病例显示圆形和泡状核，具有细颗粒染色质和小核仁，而MCPyV阴性病例显示多边形核，染色质弥散分布。实时PCR分析检测MCPyV基因显示，在默克尔细胞癌的情况下，病毒拷贝数范围为0.04-0.43/细胞。在49例Kaposi肉瘤（KS）中有3例（6.1%）也检测到MCPyV，但在192份其他疾病的DNA样本中未检测到，包括20例免疫缺陷患者的142份尸检样本。KS中MCPyV拷贝数低于默克尔细胞癌。PCR成功地从1例KS中扩增出MCPyV的全长基因组。序列分析表明，从KS分离的MCPyV与已报道的MCPyV基因组有98%的同源性。这些数据表明，MCPyV在日本的流行率较低，并且至少部分与默克尔细胞癌的发病机制相关。医学病毒学杂志81：1951-1958，2009. (C)2009 Wiley-Liss，Inc.
Merkel cell carcinoma is a rare malignancy that sometimes occurs in the skin of elderly people. Recently, a new human polyomavirus, Merkel cell polyomavirus (MCPyV) was identified in Merkel cell carcinoma. In the present study, MCPyV-DNA was detected in 6 of 11 (55%) cases of Merkel cell carcinoma by nested PCR and real-time PCR. Histologically, MCPyV-positive cases showed round and vesicular nuclei with a fine granular chromatin and small nucleoli, whereas MCPyV-negative cases showed polygonal nuclei with diffusely distributed chromatin. Real-time PCR analysis to detect the MCPyV gene revealed that viral copy numbers ranged 0.04-0.43 per cell in cases of Merkel cell carcinoma. MCPyV was also detected in 3 of 49 (6.1%) cases of Kaposi's sarcoma (KS), but not in 192 DNA samples of other diseases including 142 autopsy samples from 20 immunodeficient patients. The MCPyV copy number in KS was lower than that in Merkel cell carcinoma. PCR successfully amplified a full-length MCPyV genome from a case of KS. Sequence analysis revealed that the MCPyV isolated from KS had 98% homology to the previously reported MCPyV genomes. These data suggest that the prevalence of MCPyV is low in Japan, and is at least partly associated with the pathogenesis of Merkel cell carcinoma. J. Med. Virol. 81: 1951-1958, 2009. (C) 2009 Wiley-Liss, Inc.