Changes in Metabolism, Mitochondrial Function, and Oxidative Stress Between Female Rats Under Nonreproductive and 3 Reproductive Conditions
Changes in Metabolism, Mitochondrial Function, and Oxidative Stress Between Female Rats Under Nonreproductive and 3 Reproductive Conditions
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DOI：
10.1177/1933719118766264
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发表时间：
2019-01-01
影响因子：
2.9
通讯作者：
Kavazis, Andreas N.
中科院分区：
文献类型：
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作者：
Hyatt, Hayden W.;Zhang, Yufeng;Kavazis, Andreas N.
不哺乳的女性肥胖、二型糖尿病和癌症的发病率增加。 Stuebe 和 Rich-Edwards 提出，这些影响的发生是因为怀孕期间发生的生理变化在没有哺乳的情况下不会逆转。为了实证检验这一假设，我们比较了 4 组 Sprague-Dawley 大鼠之间的代谢、线粒体功能和氧化应激标志物：(1) 非繁殖 (NR) 大鼠，(2) 在妊娠第 20 天处死的大鼠，(3) 分娩但不允许哺乳幼崽的大鼠（非哺乳期），以及 (4) 哺乳幼崽 14 天的大鼠。与所有其他群体相比，非哺乳期女性的体脂较高。非哺乳期大鼠骨骼肌和白色脂肪组织中的过氧化物酶体增殖物激活受体δ（PPAR δ）低于其他组。 PPAR δ 与脂质代谢相关，这表明非哺乳期女性的较高脂肪量与怀孕期间设定的生理状态的保留无关，而是 PPAR δ 的独立下降。非哺乳小鼠的相对线粒体呼吸功能以及肝脏和骨骼肌的复合活性并不能预测较高的体重，并且氧化应激的测量显示组间差异最小。
Women who do not lactate display increased incidence of obesity, type II diabetes, and cancer. Stuebe and Rich-Edwards proposed that these effects occur because physiological changes that ensue during pregnancy are not reversed without lactation. To empirically test this hypothesis, we compared markers of metabolism, mitochondrial function, and oxidative stress between 4 groups of Sprague-Dawley rats: (1) nonreproductive (NR) rats, (2) rats killed at day 20 of gestation, (3) rats that gave birth but were not allowed to suckle their pups (nonlactating), and (4) rats that suckled their young for 14 days. Nonlactating females displayed higher body fat compared to all other groups. Peroxisome proliferator-activated receptor delta (PPAR delta) in skeletal muscle and white adipose tissue of nonlactating rats was lower than the other groups. The PPAR delta is associated with lipid metabolism suggesting that the higher fat mass in nonlactating females was not associated with the retention of a physiological state that was set during pregnancy but instead an independent drop in PPAR delta. Relative mitochondrial respiratory function and complex activity in the liver and skeletal muscle of nonlactating mice were not predictive of higher body mass, and measures of oxidative stress displayed minimal variation between groups.