Acetylcholine receptor clustering and nuclear movement in muscle fibers in culture.
Acetylcholine receptor clustering and nuclear movement in muscle fibers in culture.
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DOI：
10.1083/jcb.104.1.87
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发表时间：
1987-01
期刊：
影响因子：
--
通讯作者：
Rubin LL
中科院分区：
文献类型：
--
作者：
Englander LL;Rubin LL
我们研究了培养的大鼠和鸡骨骼肌细胞中乙酰胆碱受体（AChR）簇的形成和肌核的行为。这些细胞用来自电鳐电细胞外基质的因子处理，这导致它们的AChR簇的数量大量增加。我们发现，这些集群位于优先在肌细胞核以上的膜区域。这种集群核共定位解释了我们的发现，大多数的核附近的集群保持相对静止，而大多数远离集群能够在整个肌管易位。在某些情况下，首先明显形成簇，然后核迁移到下面并变得固定。如果聚集的乙酰胆碱受体后来分散，它们相关的细胞核恢复运动。这些结果表明，乙酰胆碱受体集群启动广泛的细胞骨架重排，导致亚集群定位的细胞器，潜在地提供了一个稳定的来源，新合成的乙酰胆碱受体插入到集群。
We have studied the formation of acetylcholine receptor (AChR) clusters and the behavior of myonuclei in rat and chick skeletal muscle cells grown in cell culture. These cells were treated with a factor derived from Torpedo electric extracellular matrix, which causes a large increase in their number of AChR clusters. We found that these clusters were located preferentially in membrane regions above myonuclei. This cluster-nucleus colocalization is explained by our finding that most of the nuclei near clusters remain relatively stationary, while most of those away from clusters are able to translocate throughout the myotube. In some cases, clusters clearly formed first, then nuclei migrated underneath and became immobilized. If clustered AChRs later dispersed, their associated nuclei resumed moving. These results suggest that AChR clustering initiates an extensive cytoskeletal rearrangement that causes the subcluster localization of organelles, potentially providing a stable source of newly synthesized AChRs for insertion into the cluster.