In vivo volumetric imaging of human retinal circulation with phase-variance optical coherence tomography.
In vivo volumetric imaging of human retinal circulation with phase-variance optical coherence tomography.
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DOI：
10.1364/boe.2.001504
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发表时间：
2011-06-01
影响因子：
3.4
通讯作者：
Zawadzki RJ
中科院分区：
文献类型：
--
作者：
Kim DY;Fingler J;Werner JS;Schwartz DM;Fraser SE;Zawadzki RJ
我们使用傅里叶域光学相干断层扫描（Fd-OCT）和基于相位方差的运动对比方法展示了人体视网膜微循环的体内体积图像。目前眼底荧光素血管造影（FA）是标准的技术，在临床设置可视化视网膜的血液循环。视网膜血管的高对比度成像是通过向体循环注射荧光素染料来实现的。我们之前报道了相变光学相干断层扫描（pvOCT）作为一种替代的非侵入性技术来成像人类视网膜毛细血管。与FA相比，pvOCT不仅可以无创地显示二维视网膜灌注图，还可以高灵敏度地显示视网膜微血管的体积形态。在本文中，我们报告了125 kHz的高速采集与pvOCT的a扫描，以减少运动伪影和增加扫描面积，与以前的报道相比。对不同采样密度和扫描面积的两种扫描方案进行了评估，以寻找高采集速度的体内成像的最佳参数。为了评估这项技术，我们比较了pvOCT在3x3 mm2和1.5x1.5 mm2的毛细血管成像与正常人的眼底FA。此外，介绍了视网膜毛细血管的体积视图和10个3x3 mm2 pvOCT亚体积获得的缝合图像。pvOCT对视网膜血管的可视化具有诊断视网膜血管疾病的潜力。
We present in vivo volumetric images of human retinal micro-circulation using Fourier-domain optical coherence tomography (Fd-OCT) with the phase-variance based motion contrast method. Currently fundus fluorescein angiography (FA) is the standard technique in clinical settings for visualizing blood circulation of the retina. High contrast imaging of retinal vasculature is achieved by injection of a fluorescein dye into the systemic circulation. We previously reported phase-variance optical coherence tomography (pvOCT) as an alternative and non-invasive technique to image human retinal capillaries. In contrast to FA, pvOCT allows not only noninvasive visualization of a two-dimensional retinal perfusion map but also volumetric morphology of retinal microvasculature with high sensitivity. In this paper we report high-speed acquisition at 125 kHz A-scans with pvOCT to reduce motion artifacts and increase the scanning area when compared with previous reports. Two scanning schemes with different sampling densities and scanning areas are evaluated to find optimal parameters for high acquisition speed in vivo imaging. In order to evaluate this technique, we compare pvOCT capillary imaging at 3x3 mm2 and 1.5x1.5 mm2 with fundus FA for a normal human subject. Additionally, a volumetric view of retinal capillaries and a stitched image acquired with ten 3x3 mm2 pvOCT sub-volumes are presented. Visualization of retinal vasculature with pvOCT has potential for diagnosis of retinal vascular diseases.