ALPHA-B-CRYSTALLIN IS EXPRESSED IN KIDNEY EPITHELIAL-CELL LINES AND NOT IN FIBROBLASTS
ALPHA-B-CRYSTALLIN IS EXPRESSED IN KIDNEY EPITHELIAL-CELL LINES AND NOT IN FIBROBLASTS
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DOI：
10.1016/0014-5793(89)80022-5
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发表时间：
1989-05-22
期刊：
影响因子：
3.5
通讯作者：
BHAT, SP
中科院分区：
文献类型：
--
作者：
NAGINENI, CN;BHAT, SP
我们最近发现αB结晶蛋白存在于不同胚胎起源的非眼组织中，如心脏、大脑、脊髓、肾脏、视网膜等。利用αB -晶体蛋白特异性抗血清和免疫荧光、免疫印迹、免疫沉淀和金黄色葡萄球菌蛋白酶的肽图谱，我们证实了αB -晶体蛋白在上皮细胞和成纤维细胞系中的差异表达。αB‐Crystallin仅在上皮细胞系如MDBK、MDCK、llcpk1和JTC‐12中检测到，而在两种肾成纤维细胞系、一种皮肤成纤维细胞系和一种角膜成纤维细胞系中未检测到。从这些细胞系中分离的rna的Northern blot分析也证实了αB‐晶体蛋白基因的差异表达。这些数据表明αB具有细胞类型特异性作用。
We have recently shown the presence of αB‐crystallin in non‐ocular tissues of diverse embryological origins such as the heart, brain, spinal cord, kidney, retina, etc. Using an αB‐crystallin‐specific antiserum and immunofluorescence, immunoblotting, immunoprecipitation and peptide mapping withStaphylococcus aureusprotease, we demonstrate differential expression of αB‐crystallin in epithelial and fibroblast cell lines. αB‐Crystallin was detectable only in epithelial cell lines such as MDBK, MDCK, LLCPK1and JTC‐12, and was not observed in two kidney fibroblast cell lines, one skin fibroblast cell line, and one corneal fibroblast cell line. Differential expression of the αB‐crystallin gene was also confirmed by Northern blot analysis of the RNAs isolated from these cell lines. These data suggest a cell‐type‐specific role for αB.