Genome-wide replication-independent histone H3 exchange occurs predominantly at promoters and implicates H3K56 acetylation and Asf1
Genome-wide replication-independent histone H3 exchange occurs predominantly at promoters and implicates H3K56 acetylation and Asf1
复制标题
DOI：
10.1016/j.molcel.2007.07.011
复制
发表时间：
2007-08-03
期刊：
影响因子：
16
通讯作者：
Nourani, Amine
中科院分区：
文献类型：
--
作者：
Rufiange, Anne;Jacques, Pierre-Etienne;Nourani, Amine
在酵母中，不依赖于复制的组蛋白H3/H4交换知之甚少。在这里，我们分析了沉积的组蛋白H3分子，在G1合成，使用高密度微阵列组蛋白交换试验。虽然我们发现编码区中的H3交换需要高水平的转录，但启动子在不存在转录的情况下交换H3分子。在非活性启动子中，H3主要沉积在无核小体区域周围的定位良好的核小体中，表明启动子中的一些核小体是动态的。这可能有助于抑制基因的诱导。重要的是，我们发现组蛋白H3 K56乙酰化，一个复制相关的标记，也存在于复制独立的新组装的核小体，并与新H3的沉积完全相关。最后，我们发现H3在启动子处的转录依赖性掺入高度依赖于Asfl。总之，我们的数据强调了复制独立的核小体组装/拆卸的动态性质，指定了一个链接到转录，并牵连Asfl和H3 K56乙酰化。
In yeast, histone H3/H4 exchange independent of replication is poorly understood. Here, we analyzed the deposition of histone H3 molecules, synthesized during G1, using a high-density microarray histone exchange assay. While we found that H3 exchange in coding regions requires high levels of transcription, promoters exchange H3 molecules in the absence of transcription. In inactive promoters, H3 is deposited predominantly in well-positioned nucleosomes surrounding nucleosome-free regions, indicating that some nucleosomes in promoters are dynamic. This could facilitate induction of repressed genes. Importantly, we show that histone H3 K56 acetylation, a replicationassociated mark, is also present in replicationindependent newly assembled nucleosomes and correlates perfectly with the deposition of new H3. Finally, we found that transcriptiondependent incorporation of H3 at promoters is highly dependent on Asfl. Taken together, our data underline the dynamic nature of replication-independent nucleosome assembly/disassembly, specify a link to transcription, and implicate Asfl and H3 K56 acetylation.