Effect of matrine on the expression of substance P receptor and inflammatory cytokines production in human skin keratinocytes and fibroblasts
Effect of matrine on the expression of substance P receptor and inflammatory cytokines production in human skin keratinocytes and fibroblasts
复制标题
苦参碱对人皮肤角质形成细胞和成纤维细胞P物质受体表达及炎性细胞因子产生的影响
DOI：
10.1016/j.intimp.2007.02.003
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发表时间：
2007-06-01
影响因子：
5.6
通讯作者：
Sun, Hua-Jun
中科院分区：
文献类型：
--
作者：
Liu, Ji-Yong;Hu, Jin-Hong;Sun, Hua-Jun
苦参碱是一种存在于某些槐属植物中的生物碱，在我国已广泛用于治疗病毒性肝炎、癌症、心脏病和皮肤病（如特应性皮炎和湿疹）。也已证实P物质（SP）及其受体（神经激肽-1受体，NK-1R）参与炎症性皮肤病的发病机制。因此本研究旨在探讨苦参碱对 HaCaT 细胞（人表皮角质形成细胞系）和真皮成纤维细胞中 NK-IR 表达和 SP 诱导的细胞因子产生的影响。此外，还评估了细胞活力。结果表明，苦参碱抑制HaCaT细胞和成纤维细胞中NK-IR的表达。 SP 诱导两种细胞类型产生白细胞介素 (IL)-1 beta、IL-8、干扰素 (IFN)-gamma 和单核细胞趋化蛋白 (MCP)-1。苦参碱5-100μg/mL对细胞活力影响不大。它抑制 SP 诱导的 HaCaT 细胞和成纤维细胞中 IL-1β、IL-8 和 MCP-1 的产生，同时增加 HaCaT 细胞中 IFN-γ 的产生。 SP和苦参碱均对IL-6的分泌没有影响。这些结果表明苦参碱可能通过抑制P物质受体的表达和调节炎症细胞因子的产生而对SP相关的皮肤炎症具有潜在的治疗作用。 (C) 2007 Elsevier B.V. 保留所有权利。
Matrine is a kind of alkaloid found in certain Sophora plants, which has been extensively used in China for the treatment of viral hepatitis, cancer, cardiac diseases and skin diseases (such as atopic dermatitis and eczema). It also has been confirmed that substance P (SP) and its receptor (neurokinin-1 receptor, NK-1R) are involved in the pathogenesis of inflammatory skin disorders. So the present study was designed to investigate the effect of matrine on the expression of NK-IR and cytokines production induced by SP in HaCaT cells (a human epidermal keratinocyte cell line) and dermal fibroblasts. In addition, cell viability was also evaluated. The results showed that matrine inhibited the expression of NK-IR in HaCaT cells and fibroblasts. SP induced the production of interleukin (IL)-1 beta, IL-8, interferon (IFN)-gamma, and monocyte chemotactic protein (MCP)-1 in both cell types. Matrine 5-100 mu g/mL had little effect on cell viability. It inhibited SP-induced IL-1 beta, IL-8 and MCP-1 production in HaCaT cells and fibroblasts, while it increased the production of IFN-gamma in HaCaT cells. Both SP and matrine had no effect on the secretion of IL-6. These findings suggest that matrine may have potential treatment function on SP related cutaneous inflammation by inhibition of the expression of substance P receptor and regulation of the production of inflammatory cytokines. (C) 2007 Elsevier B.V. All rights reserved.