Cloning and DNA sequencing of the surface protein antigen I/II (PAa) of Streptococcus cricetus.
Cloning and DNA sequencing of the surface protein antigen I/II (PAa) of Streptococcus cricetus.
复制标题
仓鼠链球菌表面蛋白抗原 I/II (PAa) 的克隆和 DNA 测序。
DOI：
10.1111/j.1574-6968.2001.tb10573.x
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发表时间：
2001
影响因子：
2.1
通讯作者：
H. Kato
中科院分区：
文献类型：
--
作者：
H. Tamura;T. Kikuchi;R. Shirato;H. Kato
我们克隆了a型蟋蟀链球菌E49表面蛋白抗原PAa（抗原I/II家族）的编码基因，并对其进行了序列分析。推导出的PAa氨基酸序列显示两个重复区（A区、富丙氨酸区、P区、富脯氨酸区）。在A区发现了两个额外的串联重复序列，与抗原I/II相比，P区部分缺失。同源性和系统发育分析表明，PAa与sobrinus链球菌PAg同源，而不是与变形链球菌PAc同源。利用退化PCR技术在中间链球菌中发现了一个与PAc同源的基因，而在rattus链球菌和血管链球菌中未发现。
We have cloned and sequenced the gene encoding the surface protein antigen PAa (antigen I/II family) from Streptococcus cricetus E49 (serotype a) using degenerate PCR. The deduced amino acid sequence of PAa reveals two repeating regions (A region; alanine-rich region, P region; proline-rich region). Two additional tandem repeats were found in the A region and part of the P region was deleted compared to antigen I/II. Homology and phylogenetic analyses reveal that PAa is homologous to Streptococcus sobrinus PAg rather than Streptococcus mutans PAc. Using degenerate PCR a gene homologous to PAc was identified in Streptococcus intermedius, but not found in Streptococcus rattus or Streptococcus anginosus.