Enzymic dissociation of zea shoot cell wall polysaccharides : I. Preliminary characterization of the water-insoluble fraction of zea shoot cell walls.
Enzymic dissociation of zea shoot cell wall polysaccharides : I. Preliminary characterization of the water-insoluble fraction of zea shoot cell walls.
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玉米芽细胞壁多糖的酶解：I.玉米芽细胞壁的水不溶性部分的初步表征。
DOI：
10.1104/pp.75.3.740
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发表时间：
1984
期刊：
影响因子：
7.4
通讯作者：
D. Nevins
中科院分区：
文献类型：
--
作者：
Y. Kato;D. Nevins
对玉米缓冲液匀浆的不溶性部分进行连续处理后获得的水不溶性部分。用3摩尔LiCl和热水处理杂种B73 × Mo 17的芽，用α-淀粉酶处理以除去淀粉。对该馏分进行糖组成分析，并确定主要的糖苷键。结果表明，水不溶性部分主要由阿拉伯木聚糖、（1 -> 3），（1 -> 4-）-β-d-葡聚糖、木葡聚糖、（1 -> 4）-半乳聚糖、聚半乳糖醛酸和纤维素组成。不溶性部分还含有阿魏酸（约440微克/50毫克干重），其通过用NaOH水溶液处理而从该部分中释放出来。
The water-insoluble fraction, obtained after successive treatment of an insoluble fraction of a buffer-homogenate of Zea mays L. hybrid B73 x Mo 17 shoots with 3 molar LiCl and hot water, was treated with alpha-amylase to remove starch. This fraction has been subjected to sugar composition analysis and the major glycosidic linkages were determined. The results suggest that the water-insoluble fraction is mainly composed of arabinoxylan, (1 --> 3), (1 --> 4-)-beta-d-glucan, xyloglucan, (1 --> 4)-galactan, polygalacturonic acid, and cellulose. The insoluble fraction also contained ferulic acid (about 440 micrograms per 50 milligrams dry weight), which was released from the fraction by treatment with aqueous NaOH.Novo Ban 120 from Bacillus subtilis was found to be an appropriate enzyme source for selective dissociation of Zea shoot wall polysaccharides.