Immunohistochemical analysis of the DNA methyltransferase 3b expression is associated with significant improvements in the discrimination of ulcerative colitis-associated neoplastic lesions.
Immunohistochemical analysis of the DNA methyltransferase 3b expression is associated with significant improvements in the discrimination of ulcerative colitis-associated neoplastic lesions.
复制标题
DNA 甲基转移酶 3b 表达的免疫组织化学分析与溃疡性结肠炎相关肿瘤病变辨别的显着改善相关。
DOI：
10.1007/s00595-012-0456-6
复制
发表时间：
2013
期刊：
影响因子：
2.5
通讯作者：
Fujimori T (17人中17番目)
中科院分区：
文献类型：
--
作者：
Ueda H;Fujimori T (17人中17番目)
目的明确异型增生的临床病理诊断至关重要。我们在此评估的意义，DNA甲基转移酶3b（DNMT 3b）表达作为溃疡性结肠炎（UC）相关neoplasia.MethodsThirty-one患者长期和广泛的UC的诊断标志物包括在本研究中。采用免疫组化法检测DNMT 3b在非肿瘤性直肠上皮（31例非异型增生）和结直肠肿瘤（43例异型增生和34例浸润性癌）中的表达。在具有阳性染色细胞核的细胞密度最高的区域中评估免疫反应性DNMT 3b的存在。结果直肠癌组织中DNMT 3b的表达（0.76，范围0.59-0.84）明显高于非癌组织（0.32，范围0.18- 0.67，P < 0.001）。ROC曲线分析证实0.68是肿瘤上皮中DNMT 3b表达的最佳诊断截止值（曲线下面积= 0.810）。诊断试验的敏感性为66.2%，特异性为86.7%，阳性预测值为95.7%，阴性预测值为36.1%。阳性似然比为4.98，阴性似然比为0.20。准确性为69.9%.ConclusionsA免疫组化分析的DNMT 3b表达与UC相关肿瘤病变的歧视显着改善。
PurposeMaking a clinicopathological diagnosis of dysplasia is crucial. We herein assess the significance of the DNA methyltransferase 3b (DNMT3b) expression as a diagnostic marker of ulcerative colitis (UC)-associated neoplasia.MethodsThirty-one patients with long-standing and extensive UC were included in this study. The expression of DNMT3b in non-neoplastic rectal epithelium (non-dysplasia in 31 patients) and colorectal neoplasia (dysplasia in 43 patients and invasive cancer in 34 patients) was determined using immunohistochemistry. The presence of immunoreactive DNMT3b was assessed in the areas with the highest density of cells with positively staining nuclei. DNMT3b was expressed as the percentage of positive cells relative to the total number of cells counted under high power magnification.ResultsThe DNMT3b expression in neoplastic rectal epithelium (0.76, range 0.59–0.84) was increased compared to that observed in non-neoplastic epithelium (0.32, range 0.18–0.67,P< 0.001). A ROC curve analysis confirmed 0.68 to be the best diagnostic cut-off value for the DNMT3b expression in neoplastic epithelium (area under the curve = 0.810). The sensitivity of the diagnostic test was 66.2 %, the specificity was 86.7 %, the positive predictive value was 95.7 % and the negative predictive value was 36.1 %. The positive likelihood ratio was 4.98 and the negative likelihood ratio was 0.20. The accuracy was 69.9 %.ConclusionsAn immunohistochemical analysis of the DNMT3b expression was associated with significant improvements in the discrimination of UC-associated neoplastic lesions.