Evaluation of the Cytotoxic Effects of PLGA Coated Iron Oxide Nanoparticles as a Carrier of 5-Fluorouracil and Mega-Voltage X-Ray Radiation in DU145 Prostate Cancer Cell Line
Evaluation of the Cytotoxic Effects of PLGA Coated Iron Oxide Nanoparticles as a Carrier of 5-Fluorouracil and Mega-Voltage X-Ray Radiation in DU145 Prostate Cancer Cell Line
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DOI：
10.1109/tnb.2014.2328868
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发表时间：
2014-12-01
影响因子：
3.9
通讯作者：
Mahdavi, Seied Rabi
中科院分区：
文献类型：
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作者：
Hajikarimi, Zahra;Khoei, Samideh;Mahdavi, Seied Rabi
本研究旨在探讨磁性聚乳酸-羟基乙酸共聚物（PLGA）包裹的氧化铁纳米粒作为5-氟尿嘧啶（5-FU）和X射线的载体对前列腺癌细胞系DU 145的摄取和细胞毒作用。单层培养后，DU 145细胞用不同浓度的5-FU或负载5-FU的纳米颗粒处理24小时和2Gy X射线（6兆电压（MV））。然后使用原子吸收光谱法（AAS）测量纳米颗粒渗透的速率。使用集落形成试验评价这些纳米颗粒在有/没有X射线辐射的情况下的细胞毒性效应。光谱学结果表明，铁含量，因此5-FU负载的纳米颗粒的细胞摄取随着纳米颗粒浓度的增加而增加。此外，细胞的增殖能力随着5-FU和5-FU负载的纳米颗粒浓度的增加与X射线辐射的组合而降低。然而，与游离5-FU相比，用5-FU负载的纳米颗粒与2Gy兆伏X射线辐射组合治疗后菌落数的减少程度显著更大。因此，载药纳米颗粒可以更有效地将5-FU输送到细胞中。因此，PLGA包覆的氧化铁纳米颗粒是5-FU的有效药物递送载体。PLGA涂层的氧化铁纳米颗粒是生物相容的，并且该涂层是可以渗透到细胞中的适当表面。
The purpose of this study was to investigate the uptake and cytotoxic effects of magnetic poly lactic-co-glycolic acid (PLGA)-coated iron oxide nanoparticles as a carrier of 5-fluorouracil (5-FU) and X-ray on the level of proliferation capacity of DU145 prostate carcinoma cell line in monolayer culture. Following monolayer culture, DU 145 cells were treated with different concentrations of 5-FU or 5-FU loaded nanoparticles for 24 h and 2Gy X-ray (6 Mega-voltage (MV)). The rate of nanoparticles penetration was then measured using atomic adsorption spectroscopy (AAS). The cytotoxicity effect of these nanoparticles with/without X-ray radiation was evaluated using colony formation assay. Spectroscopy results showed that iron content and therefore the cellular uptake of 5-FU loaded nanoparticles increased with increasing nanoparticle concentrations. Further, the proliferation capacity of the cells decreased with the increase of 5-FU and 5-FU loaded nanoparticle concentrations in combination with X-ray radiation. However the extent of reduction in colony number following treatment with 5-FU-loaded nanoparticles in combination with 2Gy of megavoltage X-ray radiation was significantly more than for free 5-FU. Thus, drug-loaded nanoparticles could deliver 5-FU more efficiently into the cells. PLGA coated iron oxide nanoparticles are therefore effective drug delivery vehicles for 5-FU. PLGA coated iron oxide nanoparticles are biocompatible and this coating is an appropriate surface that can penetrate into the cells.