Tuning the size of styrene-maleic acid copolymer-lipid nanoparticles (SMALPs) using RAFT polymerization for biophysical studies
Tuning the size of styrene-maleic acid copolymer-lipid nanoparticles (SMALPs) using RAFT polymerization for biophysical studies
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DOI：
10.1016/j.bbamem.2016.08.004
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发表时间：
2016-11-01
影响因子：
3.4
通讯作者：
Lorigan, Gary A.
中科院分区：
文献类型：
--
作者：
Craig, Andrew F.;Clark, Emily E.;Lorigan, Gary A.
膜蛋白的表征是具有挑战性的，由于在模仿天然脂质双层与正确折叠和功能的膜蛋白的困难。最近，苯乙烯-马来酸（StMA）共聚物已被证明有利于形成盘状的脂质双层模拟物，保持膜蛋白的结构和动态的完整性。在这里，我们报告的控制合成和表征的StMA含嵌段共聚物。合成了不同组成和分子量的StMA聚合物，并通过尺寸排阻色谱（SEC）进行了表征。这些聚合物充当1-棕榈酰-2-油酰-sn-甘油-3-磷酸胆碱（POPC）/1-棕榈酰-2-油酰-sn-甘油-3-磷酸-C（1 ′-rac-甘油（POPG）脂质的大分子表面活性剂，形成脂质的盘状结构，其中聚合物包裹在疏水脂质边缘周围。动态光散射（DLS），固态核磁共振（SSNMR）光谱和透射电子显微镜（TEM）的组合被用来表征使用这些StMA聚合物产生的纳米颗粒的大小。在1.25：1的StMA与脂质的重量比下，所产生的纳米颗粒尺寸对于2：1的比率为28 ± lnm，对于3：1的StMA比率为10 ± lnm，并且对于4：1的StMA比率为32 ± lnm，与聚合物的分子量无关。由于聚合物充当形成盘状纳米颗粒的表面活性剂，我们将这些基于StMA的嵌段共聚物称为“RAFT SMALP”。RAFT SMALPs作为一种新型的具有不同纳米尺寸的膜模拟物，可用于膜蛋白的各种生物物理研究。(C)2016由Elsevier B. V.出版
Characterization of membrane proteins is challenging due to the difficulty in mimicking the native lipid bilayer with properly folded and functional membrane proteins. Recently, styrene-maleic acid (StMA) copolymers have been shown to facilitate the formation of disc-like lipid bilayer mimetics that maintain the structural and dynamic integrity of membrane proteins. Here we report the controlled synthesis and characterization of StMA containing block copolymers. StMA polymers with different compositions and molecular weights were synthesized and characterized by size exclusion chromatography (SEC). These polymers act as macromolecular surfactants for 1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC)/1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-C(1'-rac-glycerol (POPG) lipids, forming disc like structures of the lipids with the polymer wrapping around the hydrophobic lipid edge. A combination of dynamic light scattering (DLS), solid-state nuclear magnetic resonance (SSNMR) spectroscopy, and transmission electron microscopy (TEM) was used to characterize the size of the nanoparticles created using these StMA polymers. At a weight ratio of 1.25:1 StMA to lipid, the nanoparticle size created is 28 + 1 nm for a 2:1 ratio, 10 + 1 nm for a 3:1 StMA ratio and 32 + 1 nm for a 4:1 StMA ratio independent of the molecular weight of the polymer. Due to the polymer acting as a surfactant that forms disc like nanoparticles, we term these StMA based block copolymers "RAFT SMALPs". RAFT SMALPs show promise as a new membrane mimetic with different nanoscale sizes, which can be used for a wide variety of biophysical studies of membrane proteins. (C) 2016 Published by Elsevier B.V.