Receptor-mediated stimulatory effect of oligochitosan in macrophages
Receptor-mediated stimulatory effect of oligochitosan in macrophages
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DOI：
10.1016/j.bbrc.2004.03.048
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发表时间：
2004-04-30
影响因子：
3.1
通讯作者：
Yu, QQ
中科院分区：
文献类型：
--
作者：
Feng, J;Zhao, LH;Yu, QQ
壳寡糖是以壳聚糖为原料，通过化学法或酶法水解制得的壳寡糖，其糖基残基大于3个，小于10个。在这项工作中，我们证明了壳寡糖在体外对巨噬细胞释放肿瘤坏死因子-α和白介素1-β具有刺激作用。此外，我们还通过激光共聚焦显微镜观察到壳寡糖可以被巨噬细胞摄取。Scatchard分析表明其内化是由巨噬细胞膜上的一个特异性受体介导的，其K-d为2.1×10(-5)M。竞争研究表明甘露糖能抑制壳寡糖的内化，而内毒素和β-葡聚糖不能抑制其内化。甘露糖-BSA、岩藻糖-BSA和N-乙酰氨基葡萄糖-BSA对壳寡糖内化的抑制进一步表明，壳寡糖的内化是由具有甘露糖特异性的巨噬细胞凝集素受体介导的。(C)2004 Elsevier Inc.保留所有权利。
Oligochitosan, which has greater than 3 but less than 10 saccharide (N-acetylglucosamine or glucosamine) residues, is obtained by either chemical or enzymatic hydrolysis of chitosan. In this work, we demonstrated that oligochitosan had an in vitro stimulatory effect on the release of tumor necrosis factor-alpha and interleukin-1 beta in macrophages. Moreover, we observed that oligochitosan could be uptaken by macrophages through confocal laser microscopy. Scatchard analysis of internalization of 2-aminoacridone-oligochitosan in macrophages indicated its internalization was mediated by a specific receptor on macrophage membrane with a K-d of 2.1 x 10(-5) M. Competition studies showed that mannose could inhibit oligochitosan internalization, while lipopolysaccharide and beta-glucan could not do it. Inhibition of mannose-BSA, fucose-BSA, and N-acetylglucosamine-BSA on oligochitosan internalization further suggests that oligochitosan internalization is mediated by a macrophage lectin receptor like with mannose specificity. (C) 2004 Elsevier Inc. All rights reserved.