Selective photocleavage of DNA by anthraquinone derivatives: targeting the single-strand region of hairpin structures.
Selective photocleavage of DNA by anthraquinone derivatives: targeting the single-strand region of hairpin structures.
复制标题
蒽醌衍生物对 DNA 的选择性光裂解：靶向发夹结构的单链区域。
DOI：
10.1021/bi972419g
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Schuster,GB
中科院分区：
文献类型：
--
作者：
Henderson,PT;Armitage,B;Schuster,GB
一个四聚蒽醌衍生物（27AQS2）与发夹DNA结合，照射结合的醌导致选择性的链切割。核磁共振光谱显示27AQS2结合在发夹DNA的环和茎环连接处。结合醌的紫外线照射导致环区DNA和茎区鸟嘌呤的切割。在反应混合物中加入溴化乙锭可大大提高环切割的选择性。光谱和化学证据表明该反应有三组分机制。靶向DNA结构单链区域的能力是该光核酸酶的重要特性。
A tetracationic anthraquinone derivative (27AQS2) binds to hairpin DNA and irradiation of the bound quinone leads to selective strand cleavage. NMR spectroscopy reveals that 27AQS2 binds at the loop and to the stem−loop junction of hairpin DNA. UV irradiation of the bound quinone causes cleavage of the DNA in the loop region and at guanines in the stem region. Inclusion of ethidium bromide in the reaction mixture leads to a greatly increased selectivity for loop cleavage. Spectroscopic and chemical evidence suggests a three component mechanism for reaction. The ability to target single-stranded regions of DNA structures is an important property of this photonuclease.