FRET 65: a celebration of Förster.
FRET 65: a celebration of Förster.
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FRET 65：庆祝福斯特。
DOI：
10.1117/1.jbo.17.1.011001
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发表时间：
2012
影响因子：
3.5
通讯作者：
Clegg,RobertM
中科院分区：
文献类型：
--
作者：
Periasamy,Ammasi;Vogel,StevenS;Clegg,RobertM
FRET是一个物理过程，其中能量从激发供体荧光团转移到相邻的发色团通过非辐射偶极相互作用。虽然共振能量转移最早是在20世纪20年代的荧光极化研究中观察到的，但对FRET的兴趣仅限于了解荧光去极化的浓度依赖性。现在，作为化学领域的一种好奇心，在过去的20年里，使用FRET的引用次数增加了近300倍。FRET出版物的快速增长主要源于FRET光谱和显微镜作为研究生物过程的工具，如蛋白质相互作用、构象变化、生物复合物的组装和化学计量学。这也是开发新型生物传感器的基础。重要的是，FRET成像的最新应用为应用经典的定量生化方法打开了大门，这种方法在过去50年的体外研究中非常成功，现在可以探索活细胞内的生物反应。虽然FRET最初是荧光共振能量转移的首字母缩写，但最近这个四个字母的首字母缩写被重新定义为Förster共振能量转移，以纪念西奥多Förster，他提出了定量解释这种能量转移现象的理论，并将能量转移与光合作用的基础联系起来，光合作用是生命本身的基础。Förster是一位德国物理化学家，生于1910年5月15日，卒于1974年5月20日。他在法兰克福和美因大学获得博士学位，并在波兹南州立大学担任教授。1946年，他发表了关于FRET的第一篇论文《能量迁移和荧光》。1 .本专题的目的是纪念Förster在首次发表共振能量转移论文65年后取得的成就，通过介绍最先进的FRET研究来突出FRET显微镜的发展，并庆祝相关的成像技术和试剂，如新的荧光蛋白，单分子荧光和荧光相关光谱。与FRET显微镜一起继续推动我们对生物物理学的好奇心。
FRET is a physical process where energy is transferred from an excited donor fluorophore to adjacent chromophores via nonradiative dipole interactions. While resonance energy transfer was first observed in fluorescence polarization studies in the 1920s, interest in FRET was limited to understanding the concentration dependence of fluorescence depolarization. Now, after decades as a chemical curiosity, the number of citations using FRET has increased almost 300 fold over the past 20 years. This rapid growth in FRET publications results primarily from the application of FRET spectroscopy and microscopy as a tool to study biological processes such as protein interactions, conformational changes, the assembly and stoichiometry of biological complexes. It is also the basis for the development of new biosensors. Importantly, recent applications of FRET imaging have opened the door to applying the classical quantitative biochemical approach that has been so successful over the past 50 years for in vitro studies, to now explore biological reactions inside living cells.While FRET was originally coined as an acronym for fluorescence resonance energy transfer, more recently this four-letter acronym has been redefined as Förster resonance energy transfer to honor Theodor Förster who developed the theory that quantitatively explained this energy-transfer phenomenon, and who made the connection that energy transfer was fundamental to photosynthesis, the basis of life itself. Förster was a German physical chemist, born on May 15, 1910, who died on May 20, 1974. He earned his PhD from the University of Frankfurt and Main, and was a professor at the State University of Poznan. He wrote the first publication on FRET,“Energy migration and fluorescence,” in 1946. 1 It is the purpose of this special section to honor Förster’s achievements 65 years after his first publication on resonance energy transfer, to highlight the evolution of FRET microscopy through the presentation of state-of-the-art FRET studies, and to celebrate related imaging technologies and reagents, such as new fluorescent proteins, single-molecule fluorescence, and fluorescence correlation spectroscopy, that along with FRET microscopy continue to push the envelope of our biophysical curiosity.
影响因子：
--
作者：
FORSTER, T
通讯作者：
FORSTER, T