Synchrotron FTIR micro-spectroscopy for structural analysis of Lewy bodies in the brain of Parkinson's disease patients.
Synchrotron FTIR micro-spectroscopy for structural analysis of Lewy bodies in the brain of Parkinson's disease patients.
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DOI：
10.1038/srep17625
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发表时间：
2015-12-01
影响因子：
4.6
通讯作者：
Mochizuki H
中科院分区：
文献类型：
--
作者：
Araki K;Yagi N;Ikemoto Y;Yagi H;Choong CJ;Hayakawa H;Beck G;Sumi H;Fujimura H;Moriwaki T;Nagai Y;Goto Y;Mochizuki H
路易体（Lewy bodies，LB）是帕金森病（Parkinson's disease，PD）患者的神经病理学特征，主要由α-突触核蛋白（α-synuclein，α-syn）组成。LB的精细结构是未知的，并且LB不能人工制造。然而，许多研究已经描述了使用从E.杆菌一个非常基本的问题是LB的结构是否与重组淀粉样蛋白原纤维的结构相同。因此，我们使用同步辐射傅里叶变换红外显微光谱（FTIRM）来分析PD患者脑中LB的精细结构。我们的研究结果表明，红外光谱的位移表明LB中富含β-片层结构。此外，LB的二维红外成像显示，β-折叠结构的含量在晕中比在核心中高，并且核心含有大量的蛋白质和脂质。
Lewy bodies (LBs), which mainly consist of α-synuclein (α-syn), are neuropathological hallmarks of patients with Parkinson’s disease (PD). The fine structure of LBs is unknown, and LBs cannot be made artificially. Nevertheless, many studies have described fibrillisation using recombinant α-syn purified from E. coli. An extremely fundamental problem is whether the structure of LBs is the same as that of recombinant amyloid fibrils. Thus, we used synchrotron Fourier transform infrared micro-spectroscopy (FTIRM) to analyse the fine structure of LBs in the brain of PD patients. Our results showed a shift in the infrared spectrum that indicates abundance of a β-sheet-rich structure in LBs. Also, 2D infrared mapping of LBs revealed that the content of the β-sheet structure is higher in the halo than in the core, and the core contains a large amount of proteins and lipids.