Association of heat shock proteins and neuronal membrane components with lipid rafts from the rat brain
Association of heat shock proteins and neuronal membrane components with lipid rafts from the rat brain
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DOI：
10.1002/jnr.20575
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发表时间：
2005-08-15
影响因子：
4.2
通讯作者：
Brown, IR
中科院分区：
文献类型：
--
作者：
Chen, S;Bawa, D;Brown, IR
脂筏是一种特殊的质膜微结构域，富含胆固醇和鞘脂，是信号转导复合物的主要组装和分选平台。从大鼠前脑和小脑分离的脂筏中存在组成性表达的热休克蛋白Hsp90、Hsc70、Hsp60和Hsp40以及一系列神经递质受体。胆固醇的消耗使这些蛋白质与脂筏分离。在高温胁迫后，脂筏标记蛋白flotilin -1的水平没有明显变化。热疗后1小时在脂筏中检测到应激诱导的Hsp70，并在24小时达到峰值，提示Hsp70可能在神经应激后维持脂筏相关信号转导复合物的稳定性中发挥作用。(c) 2005 Wiley-Liss, Inc。
Lipid rafts are specialized plasma membrane microdomains enriched in cholesterol and sphingolipids that serve as major assembly and sorting platforms for signal transduction complexes. Constitutively expressed heat shock proteins Hsp90, Hsc70, Hsp60, and Hsp40 and a range of neurotransmitter receptors are present in lipid rafts isolated from rat forebrain and cerebellum. Depletion of cholesterol dissociates these proteins from lipid rafts. After hyperthermic stress, flotillin-1, a lipid raft marker protein, does not show major change in levels. Stress-inducible Hsp70 is detected in lipid rafts at 1 hr posthyperthermia, with the peak levels attained at 24 hr, suggesting that Hsp70 may play roles in maintaining the stability of lipid raft-associated signal transduction complexes following neural stress. (c) 2005 Wiley-Liss, Inc.