A system to reproduce and quantify the biomechanical environment of the cell.
A system to reproduce and quantify the biomechanical environment of the cell.
复制标题
DOI：
10.1152/jappl.1989.67.1.397
复制
发表时间：
1989-07
影响因子：
3.3
通讯作者：
F. K. Winston;E. Macarak;S. Gorfien;Lawrence E. Thibault
中科院分区：
文献类型：
--
作者：
F. K. Winston;E. Macarak;S. Gorfien;Lawrence E. Thibault
已经开发出一种体外系统，可以高精度地对细胞群施加均匀的生物力学刺激。该装置设计用于使活细胞承受从准静态到 1 s-1 的速率和从 0 到 5 Hz 的频率的可重复和可量化的 0 到 10% 的双轴应变。基于易于测量的参数，导出并通过实验验证了用于确定细胞生长的基质中的应变的方程。测定了基质的机械性能，并证明细胞可以很容易地在该装置中培养。利用该系统，对克隆牛肺动脉内皮细胞克隆进行5%双轴应变，峰值应变率为0.5 s-1，频率为1 Hz，持续7小时，细胞活力大于84%，细胞脱离小于8%。我们证明细胞必须附着在基底上才能被拉伸，并且细胞应变和基底应变不相等。使用荧光标记的珠子作为细胞表面标记来测量由于基底变形而在细胞中产生的实际应变，发现细胞伸长约为基底中应变的 60%。该常数似乎受到体外细胞年龄和形态的影响。
An in vitro system that permits application of a uniform biomechanical stimulus to a population of cells with great precision has been developed. The device is designed to subject living cells to reproducible and quantifiable biaxial strains from 0 to 10% at rates from quasi-static to 1 s-1 and frequencies from 0 to 5 Hz. Equations for determining the strain in the substrate upon which the cells are grown, based on easily measured parameters, are derived and validated experimentally. The mechanical properties of the substrate are determined, and it is demonstrated that cells can easily be cultured in the apparatus. By use of the system, cloned bovine pulmonary artery endothelial cell clones are subjected to 5% biaxial strains applied at a peak strain rate of 0.5 s-1 and a frequency of 1 Hz for 7 h with cell viability greater than 84% and cell detachment less than 8%. We demonstrate that cells must be attached to the substrate for them to be stretched and that cell strain and substrate strain are not equal. With the use of fluorescently labeled beads as cell surface markers to measure the actual strain produced in the cells as a result of the deformation of the substrate, cell elongation was found to be approximately 60% of the strain in the substrate. This constant appeared to be affected by both in vitro cell age and morphology.