Ultraviolet-induced reactivation, amplification, and hypomethylation of a herpes simplex virus thymidine kinase gene.
Ultraviolet-induced reactivation, amplification, and hypomethylation of a herpes simplex virus thymidine kinase gene.
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紫外线诱导单纯疱疹病毒胸苷激酶基因的再激活、扩增和低甲基化。
DOI：
10.1007/bf01535228
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Clough,DW
中科院分区：
文献类型：
--
作者：
DeVries,PJ;Davidson,RL;Clough,DW
用紫外线（UV）照射含有甲基化和失活的单纯疱疹病毒胸苷激酶（TK）基因的小鼠细胞系，试图诱导失活TK基因的表达。紫外线照射能够以剂量依赖性方式诱导viralTK基因的表达。对viralTK基因甲基化模式的分析表明，在三个UV诱导的TK+细胞系中，活性TK基因的甲基化程度低于亲本细胞中的非活性viralTK基因。在亲本和UV诱导的细胞系中的viralTK基因的拷贝数的分析还显示，在所测试的四个UV诱导的TK+细胞系中的三个中，viralTK基因扩增3- 20倍。
A line of mouse cells containing a methylated and inactive herpes simplex virus thymidine kinase(TK)gene was irradiated with ultraviolet (UV) light in an attempt to induce expression of the inactiveTKgene. UV irradiation was shown to be capable of inducing expression of the viralTKgene in a dose-dependent manner. Analysis of the methylation pattern of the viralTKgene indicated that the activeTKgene in three UV-induced TK+cell lines was methylated to a lesser extent than was the inactive viralTKgene in the parental cells. Analysis of the copy number of the viralTKgene in parental and UV-induced cell lines also revealed that the viralTKgene was amplified 3- to 20-fold in three of four UV-induced TK+cell lines tested.