Monozygotic twin formation in mouse embryos in vitro.
Monozygotic twin formation in mouse embryos in vitro.
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DOI：
10.1126/science.7190325
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发表时间：
1980-08
期刊：
影响因子：
56.9
通讯作者：
Y. Hsu;M. Gonda
中科院分区：
文献类型：
--
作者：
Y. Hsu;M. Gonda
单卵双胞胎从培养的小鼠囊胚中以约1：100的比例发育而来。裸露的囊胚附着在培养皿上的部位通常是其壁滋养层的随机切片，在这种情况下，单个卵圆柱体单侧发育。然而，在那些少数的囊胚附着其反极性壁滋养层，内细胞团成为细分为两个部分，因为施加限制其生长的顶部位于极性滋养层和塑料基板。每个细分显然纳入全能细胞，导致在双边形成的两个卵柱共享相同的外胎盘锥。
Monozygotic twins developed from cultured murine blastocysts at the ratio of approximately 1:100. The locus at which the denuded blastocysts attached to the culture dish was usually a random section of their mural trophoblasts, in which case single egg cylinders developed unilaterally. However, in those few blastocysts attaching with their antipolar mural trophoblasts, the inner cell mass became subdivided into two parts because of restrictions imposed on its growth by the apically situated polar trophoblasts and the plastic substrate. Each subdivision apparently incorporated totipotent cells, resulting in the bilateral formation of two egg cylinders sharing the same ectoplacental cone.