Measurement of kinase activation in single mammalian cells
Measurement of kinase activation in single mammalian cells
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DOI：
10.1038/73760
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发表时间：
2000-03-01
影响因子：
46.9
通讯作者：
Allbritton, NL
中科院分区：
文献类型：
--
作者：
Meredith, GD;Sims, CE;Allbritton, NL
我们展示了一种同时测量单细胞内关键调节酶激活的新方法。为了说明这项技术的能力，我们测量了蛋白激酶C(PKC)、蛋白激酶A(PKA)、钙-钙调蛋白激活蛋白激酶II(CaMKII)和cdc2蛋白激酶(Cdc2K)在药物刺激或生理刺激下的激活情况。这一检测策略应该适用于广泛的细胞内酶，包括磷酸酶、蛋白水解酶、核酸酶和其他激动酶。
We demonstrate a new method for the simultaneous measurement of the activation of key regulatory enzymes within single cells. To illustrate the capabilities of the technique, the activation of protein kinase C (PKC), protein kinase A (PKA), calcium-calmodulin activated kinase II (CamKII), and cdc2 protein kinase (cdc2K) was measured in response to both pharmacological or physiological stimuli. This assay strategy should be applicable to a broad range of intracellular enzymes, including phosphatases, proteases, nucleases, and other kinases.