In vitro drug susceptibility of 2275 clinical non-tuberculous Mycobacterium isolates of 49 species in The Netherlands
In vitro drug susceptibility of 2275 clinical non-tuberculous Mycobacterium isolates of 49 species in The Netherlands
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DOI：
10.1016/j.ijantimicag.2009.09.023
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发表时间：
2010-02-01
影响因子：
10.8
通讯作者：
van Soolingen, Dick
中科院分区：
文献类型：
--
作者：
van Ingen, Jakko;van der Laan, Tridia;van Soolingen, Dick
在这项研究中，使用 Middlebrook 7H10 琼脂稀释法对荷兰分离的 49 种非结核分枝杆菌的 2275 株临床分离株进行了标准化药敏试验。克拉霉素和利福布丁最为活跃，分别有 87% 和 83% 的分离株敏感。对环丙沙星 (44%) 和阿米卡星 (32%) 的敏感性有限，主要限于堪萨斯分枝杆菌、爪蟾分枝杆菌、偶然分枝杆菌和系统发育相关物种。对异烟肼（0.5%）、利福平（37%）、乙胺丁醇（35%）和链霉素（33%）的敏感性很少见；对环丝氨酸、氯法齐明和丙硫异烟胺的敏感性通常仅限于生长缓慢的植物，尽管丙硫异烟胺也具有对抗偶发分枝杆菌和相关物种的活性。体外和体内活性之间存在显着差异。为了提高药敏试验的实用性，药物的选择应改为更多具有已证实的临床疗效且与体外药敏相关的药物。 (C) 2009 Elsevier B.V. 和国际化疗协会。版权所有。
In this study, 2275 clinical isolates of 49 species of non-tuberculous mycobacteria isolated in The Netherlands were subjected to standardised drug susceptibility testing using the Middlebrook 7H10 agar dilution method. Clarithromycin and rifabutin were most active, with 87% and 83% of all isolates, respectively, being susceptible. Susceptibility to ciprofloxacin (44%) and amikacin (32%) was limited and was mostly restricted to Mycobacterium kansasii, Mycobacterium xenopi, Mycobacterium fortuitum and phylogenetically related species. Susceptibility to isoniazid (0.5%), rifampicin (37%), ethambutol (35%) and streptomycin (33%) was rare; susceptibility to cycloserine, clofazimine and prothionamide was generally restricted to slow growers, although prothionamide also had activity against M. fortuitum and related species. Significant discrepancies between in vitro and in vivo activity exist. To improve the utility of drug susceptibility testing, the selection of drugs should be changed to more drugs with proven clinical efficacy correlating with in vitro susceptibility. (C) 2009 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.