Alterations in steroid hormone production by porcine ovarian granulosa cells caused by bisphenol A and bisphenol A dimethacrylate
Alterations in steroid hormone production by porcine ovarian granulosa cells caused by bisphenol A and bisphenol A dimethacrylate
复制标题
DOI：
10.1016/j.mce.2005.02.009
复制
发表时间：
2005-12-01
影响因子：
4.1
通讯作者：
Scsuková, S
中科院分区：
文献类型：
--
作者：
Mlynarcíková, A;Kolena, J;Scsuková, S
我们研究了内分泌干扰物双酚A （BPA）和BPA-二甲丙烯酸酯（BPA- dma）作为增塑剂，在猪卵巢颗粒细胞孵育72小时后对类固醇激素产生的影响。10(-8) M ~ 10(-5) NI双酚a使基础孕酮水平升高，而相同浓度范围的双酚a - dma没有引起任何变化。在fsh刺激细胞后，BPA-DMA显示出抑制孕酮产生的趋势。然而，在10(-7)M和10(-6)M浓度下，BPA甚至能够扩增fsh刺激的黄体酮合成。BPA和BPA- dma在整个浓度范围内抑制fsh诱导的雌二醇生成。在10(-8)M至10(-5)M期间，BPA没有改变lh刺激的黄体酮产生，而BPA- dma降低了培养基中的黄体酮水平。在10(-4)M浓度下，两种被测试的药物对黄体酮的产生有显著的抑制作用，特别是在基础和促性腺激素刺激下。提示卵巢甾体生成可能是双酚a和双酚a - dma干扰内分泌的可能部位之一。2005爱思唯尔爱尔兰有限公司版权所有。
We have investigated the effects of bisphenol A (BPA) and BPA-dimethacrylate (BPA-DMA), endocrine disruptors used as plasticizers, on steroid hormone production by porcine ovarian granulosa cells after 72 h incubation. BPA at 10(-8) M to 10(-5) NI increased basal progesterone levels, while the same concentration range of BPA-DMA did not cause any changes. After FSH-stimulation of the cells, BPA-DMA showed a tendency to inhibit progesterone production. BPA, however, at 10(-7) M and 10(-6) M concentrations was even able to amplify FSH-stimulated progesterone synthesis. BPA as well as BPA-DMA inhibited FSH-induced estradiol production in the whole concentration range. LH-stimulated progesterone production was not altered by BPA in 10(-8) M to 10(-5) M, while BPA-DMA decreased progesterone levels in the cultured media. Significant inhibitory effect of both tested agents at 10(-4) M concentrations was observed specifically on progesterone production, basal as well as gonadotropin-stimulated. The results indicate that ovarian steroidogenesis might be one of the possible sites afflicted by the endocrine disrupting action of BPA and BPA-DMA. (c) 2005 Elsevier Ireland Ltd. All rights reserved.