The dnd RNA Identifies Germ Cell Origin and Migration in Olive Flounder (Paralichthys olivaceus)
The dnd RNA Identifies Germ Cell Origin and Migration in Olive Flounder (Paralichthys olivaceus)
复制标题
dnd RNA 鉴定橄榄比目鱼 (Paralichthys olivaceus) 生殖细胞的起源和迁移
DOI：
10.1155/2015/428591
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发表时间：
2015-01-01
影响因子：
--
通讯作者：
Li, Jun
中科院分区：
文献类型：
--
作者：
Wang, Xueying;Liu, Qinghua;Li, Jun
The present study obtained a germ cell-specific marker dead end (dnd) in olive flounder (Paralichthys olivaceus) named Podnd. The tissue-specific expressions of Podnd transcripts were present in testis and ovary but were not detectable in other somatic tissues detected. SISH showed that Podnd expressed only in germ cells at different developmental stages but not in surrounding somatic cells. The expression of Podnd during embryonic development at 16 different stages revealed that the relative expression of Podnd transcript fluctuated at a high level in the cleavage stages, gradually decreased through subsequent development, and reached the lowest at late gastrula stage till it was nearly undetectable. The Podnd transcripts localization and migration were similar to zebrafish. Further research on the specification migration mechanism of PGCs and the role of germ cell during gonadal development in olive flounder would improve our understanding of germline development.
