Characterization of a large glycoprotein proteoglycan by size-exclusion chromatography combined with light and X-ray scattering methods
Characterization of a large glycoprotein proteoglycan by size-exclusion chromatography combined with light and X-ray scattering methods
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通过尺寸排阻色谱法结合光和 X 射线散射方法表征大糖蛋白蛋白多糖
DOI：
10.1016/j.chroma.2013.06.048
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发表时间：
2013
影响因子：
4.1
通讯作者：
Yoji Inoko
中科院分区：
文献类型：
--
作者：
Yasushi Watanabe;Yoji Inoko
通过尺寸排阻色谱法结合低角激光散射和小角 X 射线散射方法，对溶液中源自鲨鱼软骨的蛋白多糖的分子量和链构象进行了表征。蛋白多糖的总分子量为3.9±20万，主要成分的分子量约为2.0±20万。 X射线散射数据显示，蛋白多糖的主要成分几乎等于排除体积的链，其持续长度范围为13.5至16.4 nm。这些结果表明，尺寸排阻色谱法与小角度激光散射和小角度 X 射线散射测量相结合，对于溶液中大型生物聚合物的表征具有互补作用。
The molecular weight and chain conformation of a proteoglycan derived from shark cartilage in solution were characterized by size-exclusion chromatography combined with low-angle laser light scattering and small-angle X-ray scattering methods. The total molecular weight of the proteoglycan was 3.9 ± 0.2 million and the molecular weight of the main component was about 2.0 ± 0.2 million. The X-ray scattering data revealed that the main components of the proteoglycan are nearly equal to a chain with excluded volume and their persistence lengths range from 13.5 to 16.4 nm. These results show that size-exclusion chromatography combined with low-angle laser light scattering and small-angle X-ray scattering measurements are complementarily useful for characterization of large biopolymers in solution.