Molecular characterization and transcription of the luxS gene that encodes LuxS autoinducer 2 synthase in Streptococcus bovis
Molecular characterization and transcription of the luxS gene that encodes LuxS autoinducer 2 synthase in Streptococcus bovis
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DOI：
10.1007/s00284-004-4356-x
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发表时间：
2004-11-01
影响因子：
2.6
通讯作者：
Hino, T
中科院分区：
文献类型：
--
作者：
Asanuma, N;Yoshii, T;Hino, T
证明了牛链球菌中存在编码LuxS自诱导物2（AI-2）合酶的luxS基因，并检测了该基因的分子特性和转录。色葡萄bovis luxS以单顺反子方式转录。细胞内luxS-mRNA在指数生长初期急剧增加，指数生长中期后急剧下降。luxS转录的大幅下降在向细胞供应的葡萄糖减少或生长速率下降之前开始。luxS的转录与细胞密度没有直接关系，当细胞以最大速率生长时，luxS的转录以最大速率继续。可以设想，S.牛作为响应环境条件调节细胞生理和代谢的信号。然而，LuxS在S.牛，包括AI-2合成的调节，仍有待澄清。
Presence of the luxS gene that encodes LuxS autoinducer 2 (AI-2) synthase in Streptococcus bovis was demonstrated, and the molecular properties and transcription of the gene were examined. The S. bovis luxS was transcribed in a monocistronic fashion. Intracellular luxS-mRNA increased sharply during the initial exponential growth, and decreased abruptly after the middle exponential phase. The large drop in luxS transcription began before the glucose supply to cells decreased or the growth rate declined. Transcription of luxS was not directly related to cell density, and continued at a maximal rate when cells were kept growing at a maximal rate. It is conceivable that AI-2 activity in S. bovis acts as a signal for adjusting cell physiology and metabolism in response to environmental conditions. However, the role of LuxS in S. bovis, including the regulation of AI-2 synthesis,, remains to be clarified.