A surface plasmon resonance-based immunosensor for highly sensitive detection of morphine
A surface plasmon resonance-based immunosensor for highly sensitive detection of morphine
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DOI：
10.1016/s0925-4005(98)00107-5
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发表时间：
1998-06-25
影响因子：
8.4
通讯作者：
Yamazoe, N
中科院分区：
文献类型：
--
作者：
Sakai, G;Ogata, K;Yamazoe, N
利用抗吗啡（MO）单克隆抗体和MO-牛血清白蛋白（MO-BSA）偶联物（抗原）实现了基于表面等离子体共振（SPR）的高灵敏度和高选择性检测。采用物理吸附法将MO-BSA固定在SPR传感器芯片的Au薄膜上。当抗体浓度达到约100 μ mol/L时，表面等离子体共振系统的入射角随抗体浓度的增加几乎呈线性增加。5 μ g ml（-1）（ppm）。发现向抗体溶液中添加MO（5 ppm）由于MO的抑制作用而急剧减小入射角偏移。基于这种抑制原理，本传感器可以检测浓度范围为0.1-10 ng ml（-1）（ppb）的MO。抗体和抗原（MO和MO-BSA）之间的亲和常数可以通过假定免疫反应系统的Langmuir吸附模型获得。(C)1998年Elsevier Science S.A. All rights reserved.
Highly sensitive and selective detection of morphine (MO) based on surface-plasmon-resonance (SPR) was realized by using an anti-MO monoclonal antibody and MO-bovine serum albumin (MO-BSA) conjugate (antigen). MO-BSA was immobilized on the Au thin film of the SPR sensor chip by physical adsorption. The incident angle of the SPR system using the MO-BSA immobilized chip increased almost linearly with increasing concentration of antibody up to ca. 5 mu g ml(-1) (ppm). The addition of MO to the antibody solution (5 ppm) was found to reduce the incident angle shift sharply because of the inhibition effect of MO. Based on this inhibiting principle, the present sensor could detect MO in the concentration range 0.1-10 ng ml(-1) (ppb). The affinity constants between the antibody and the antigens (MO and MO-BSA) could be obtained by assuming a Langmuir adsorption model for the immunoreaction system. (C) 1998 Elsevier Science S.A. All rights reserved.